The requirement of ubiquitin C-terminal hydrolase L1 in mouse ovarian development and fertility†.
The requirement of ubiquitin C-terminal hydrolase L1 in mouse ovarian development and fertility†.
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DOI：
10.1093/biolre/ioac086
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发表时间：
2022-08-09
影响因子：
3.6
通讯作者：
Grive, Kathryn J.
中科院分区：
文献类型：
--
作者：
Woodman, Morgan F.;Ozcan, Meghan C. H.;Gura, Megan A.;De La Cruz, Payton;Gadson, Alexis K.;Grive, Kathryn J.
泛素C-末端水解酶L1（UCHL 1）是一种富含于神经元和性腺组织中的去泛素化酶，已知其调节单泛素的细胞储存和蛋白质周转。虽然它在维持适当的运动神经元功能方面的功能已经得到了很好的确立，但对其在生殖过程的健康和功能方面的作用的研究才刚刚开始。对小鼠围产期所有卵巢细胞的单细胞测序分析显示，Uchl 1在发育中的卵母细胞群体中表达非常高，这一观察结果被小鼠生殖寿命中所有阶段的卵母细胞中高水平的卵母细胞富集的UCHL 1蛋白表达所证实。为了更好地理解UCHL 1可能在卵母细胞中发挥的作用，我们使用了UCHL 1缺陷小鼠系，发现与野生型和杂合子同窝仔相比，这些动物的窝仔数减少，窝仔数减少，卵泡发生改变，卵母细胞形态异常，发情周期中断和明显的内分泌功能障碍。这些数据揭示了UCHL 1在女性生育力和整体卵巢功能中的新作用，并表明泛素蛋白酶体途径在介导生殖健康中的潜在重要作用。泛素C末端水解酶L1（UCHL 1）是雌性小鼠正常卵泡发生、发情周期和生育力所必需的。
Ubiquitin C-terminal hydrolase L1 (UCHL1) is a de-ubiquitinating enzyme enriched in neuronal and gonadal tissues known to regulate the cellular stores of mono-ubiquitin and protein turnover. While its function in maintaining proper motor neuron function is well established, investigation into its role in the health and function of reproductive processes is only just beginning to be studied. Single-cell-sequencing analysis of all ovarian cells from the murine perinatal period revealed that Uchl1 is very highly expressed in the developing oocyte population, an observation which was corroborated by high levels of oocyte-enriched UCHL1 protein expression in oocytes of all stages throughout the mouse reproductive lifespan. To better understand the role UCHL1 may be playing in oocytes, we utilized a UCHL1-deficient mouse line, finding reduced number of litters, reduced litter sizes, altered folliculogenesis, morphologically abnormal oocytes, disrupted estrous cyclicity and apparent endocrine dysfunction in these animals compared to their wild-type and heterozygous littermates. These data reveal a novel role of UCHL1 in female fertility as well as overall ovarian function, and suggest a potentially essential role for the ubiquitin proteasome pathway in mediating reproductive health. Ubiquitin C-terminal hydrolase L1 (UCHL1) is required for proper ovarian folliculogenesis, estrous cyclicity, and fertility in the female mouse.
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影响因子：
2.4
作者：
Kwon, J;Kikuchi, T;Yoshikawa, Y
通讯作者：
Yoshikawa, Y
影响因子：
6.1
作者：
Liu, Hao;Li, Wenjin;Ahmad, Muzamil;Miller, Tricia M.;Rose, Marie E.;Poloyac, Samuel M.;Uechi, Guy;Balasubramani, Manimalha;Hickey, Robert W.;Graham, Steven H.
通讯作者：
Graham, Steven H.
体内调节哺乳动物原始卵泡激活的因素和途径。
影响因子：
5.5
作者：
Chen Y;Yang W;Shi X;Zhang C;Song G;Huang D
通讯作者：
Huang D
DOI：
10.1042/bcj20160082
发表时间：
2016-08-15
期刊：
The Biochemical journal
影响因子：
--
作者：
Bishop P;Rocca D;Henley JM
通讯作者：
Henley JM
影响因子：
6
作者：
Alpaugh WF;Voigt AL;Dardari R;Su L;Al Khatib I;Shin W;Goldsmith TM;Coyle KM;Tang LA;Shutt TE;Klein C;Biernaskie J;Dobrinski I
通讯作者：
Dobrinski I