Correlation of 3-mercaptopropionic acid induced seizures and changes in striatal neurotransmitters monitored by microdialysis.
Correlation of 3-mercaptopropionic acid induced seizures and changes in striatal neurotransmitters monitored by microdialysis.
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DOI：
10.1016/j.ejps.2013.11.019
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发表时间：
2014-06-16
影响因子：
4.6
通讯作者：
Lunte, Craig E.
中科院分区：
文献类型：
--
作者：
Crick, Eric W.;Osorio, Ivan;Frei, Mark;Mayer, Andrew P.;Lunte, Craig E.
关键词：
本研究的目的是在大鼠中使用惊厥剂3-巯基丙酸（3-MPA）的癫痫持续状态稳态化学模型，并比较慢（5分钟）和快（60秒）时间尺度上纹状体神经传递的变化。在体内微透析结合电生理方法，以提供一个完整的评价所获得的结果的动力学。比较稳态化学模型癫痫持续状态对纹状体氨基酸和胺类神经递质含量的影响，通过在体微透析结合电生理方法测量。对每60秒和每5分钟收集的样品进行测量。“快”（60秒）和“慢”（5分钟）选择采样时间尺度，以获得对GABA合成抑制的动力学及其对其他神经递质和皮层电活动的影响的更多了解。3-MPA的静脉内负荷（60毫克/公斤），然后通过一个恒定的输注（50毫克/公斤/分钟）分钟的形式管理。微透析样品收集从纹状体的间隔为5分钟和60秒，并分析生物胺和氨基酸神经递质。通过放置在皮质上的螺钉监测ECoG活动。在5分钟的样本中，谷氨酸（Glu）增加，γ-氨基丁酸（GABA）减少，而多巴胺（DA）浓度的变化呈双峰。在60秒样品中，Glu变化是双峰的，这是5分钟样品不明显的特征。ECoG活动提示癫痫持续状态。本研究描述了在体微透析与电生理学相结合，以监测3-MPA对大脑神经传递的影响。这导致了更好地理解纹状体的化学变化，由于应用3-MPA癫痫持续状态的化学模型。
The goal of this study was to use a status epilepticus steady-state chemical model in rats using the convulsant, 3-mercaptopropionic acid (3-MPA), and to compare the changes in striatal neurotransmission on a slow (5 minute) and fast (60 second) timescale. In vivo microdialysis was combined with electrophysiological methods in order to provide a complete evaluation of the dynamics of the results obtained. To compare the effects of a steady-state chemical model pof status epilepticus on striatal amino-acid and amine neurotransmitters contents, as measured via in vivo microdialysis combined with electrophysiological methods. Measurements were performed on samples collected every 60 seconds and every 5 minutes. “Fast” (60s) and “slow” (5 min.) sampling timescales were selected, to gain more insight into the dynamics of GABA synthesis inhibition and of its effects on other neurotransmitters and on cortical electrical activity. 3-MPA was administered in the form of an intra-venous load(60 mg/kg) followed by a constant infusion (50 mg/kg/min) for min. Microdialysis samples were collected from the striatum at intervals of 5 minutes and 60 seconds and analyzed for biogenic amine and amino acid neurotransmitters. ECoG activity was monitored via screws placed over the cortex. In the 5 minute samples, glutamate (Glu) increased and γ-aminobutyric acid (GABA) decreased monotonically while changes in dopamine (DA) concentration were bimodal. In the sixty second samples, Glu changes were bimodal, a feature that was not apparent with the five minute samples. ECoG activity was indicative of status epilepticus. This study describes the combination of in vivo microdialysis with electrophysiology to monitor the effect of 3-MPA on neurotransmission in the brain. This led to a better understanding of the chemical changes in the striatum due to the applied 3-MPA chemical model of status epilepticus.
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