Monocyte Chemotactic Factor in Rheumatoid Arthritis Synovial Tissue
Monocyte Chemotactic Factor in Rheumatoid Arthritis Synovial Tissue
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类风湿关节炎滑膜组织中的单核细胞趋化因子
DOI：
--
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发表时间：
1996
影响因子：
4.8
通讯作者：
Tetsurou Yamamoto
中科院分区：
文献类型：
--
作者：
H. Nishiura;Y. Shibuya;S. Matsubara;S. Tanase;T. Kambara;Tetsurou Yamamoto
类风湿关节炎滑膜病变的提取物从七个病人具有很强的趋化活性的单核细胞和一个可以忽略不计的多形核白细胞。这些结果与滑膜病变的显著组织学特征，即单核细胞为主的浸润一致。滑膜组织提取物中的主要单核细胞趋化因子在具有C4柱的反相高效液相色谱中被纯化为单一蛋白峰。起始20个残基的NH-末端氨基酸分析产生单一序列。令人惊讶的是，该序列与S19核糖体蛋白的序列完全相同。纯化的样品在SDS-聚丙烯酰胺凝胶电泳中显示两条蛋白带，其表观分子量为34和68 kDa。这些大小分别是S19核糖体蛋白的2倍和4倍，表明趋化因子是由因子XIIIa交联的S19核糖体蛋白的二聚体或四聚体。重组人S19核糖体蛋白在大肠杆菌中被制备为与麦芽糖结合蛋白的融合蛋白。在用因子XIIIa处理后，交联的重组S19核糖体蛋白表现出单核细胞趋化活性，尽管未处理的重组蛋白没有。
The extracts of rheumatoid arthritis-synovial lesions from seven patients possessed a strong chemotactic activity for monocytes and a negligible one for polymorphonuclear leukocytes. These results are consistent with a prominent histological feature of the synovial lesion, the mononuclear cell predominant infiltration. The major monocyte chemotactic factor in the synovial tissue extracts was purified to a single protein peak in reverse phase high performance liquid chromatography with a C4 column. NH-terminal amino acid analysis of the initial 20 residues yielded a single sequence. Surprisingly, this sequence was completely identical to that of S19 ribosomal protein. The purified sample demonstrated two protein bands in SDS-polyacrylamide gel electrophoresis with apparent molecular masses of 34 and 68 kDa. These sizes were 2 and 4 times that of S19 ribosomal protein, suggesting that the chemotactic factor would be a dimer or tetramer of S19 ribosomal protein cross-linked by factor XIIIa. A recombinant human S19 ribosomal protein was prepared as a fusion protein with a maltose binding protein in Escherichia coli. After treatment with factor XIIIa, cross-linked recombinant S19 ribosomal protein exhibited the monocyte chemotactic activity, although the untreated recombinant protein did not.
单核细胞对趋化刺激的反应性是可以对多种趋化剂作出反应的细胞亚群的特性。
DOI：
10.1172/jci110033
发表时间：
1981
期刊：
The Journal of clinical investigation
影响因子：
--
作者：
Cianciolo,GJ;Snyderman,R
通讯作者：
Snyderman,R
人类皮肤中真皮树突状细胞和肥大细胞之间的新相互作用。
DOI：
--
发表时间：
1993
期刊：
Laboratory investigation; a journal of technical methods and pathology
影响因子：
--
作者：
Sueki,H;Whitaker,D;Buchsbaum,M;Murphy,GF
通讯作者：
Murphy,GF
人类甲酰肽受体的一系列六种配体：具有高趋化效力和功效的四肽。
DOI：
10.1073/pnas.84.22.7967
发表时间：
1987
影响因子：
11.1
作者：
Rot,A;Henderson,LE;Copeland,TD;Leonard,EJ
通讯作者：
Leonard,EJ