Identification of multiple subsets of ventral interneurons and differential distribution along the rostrocaudal axis of the developing spinal cord.
Identification of multiple subsets of ventral interneurons and differential distribution along the rostrocaudal axis of the developing spinal cord.
复制标题
DOI：
10.1371/journal.pone.0070325
复制
发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Clotman F
中科院分区：
文献类型：
--
作者：
Francius C;Harris A;Rucchin V;Hendricks TJ;Stam FJ;Barber M;Kurek D;Grosveld FG;Pierani A;Goulding M;Clotman F
脊髓包含称为中枢模式发生器（CPG）的神经元回路，可协调节律性运动活动。 CPG 回路由运动神经元和多种中间神经元细胞类型组成，其中许多细胞源自四种不同的腹侧中间神经元基本类别，称为 V0、V1、V2 和 V3。虽然在阐明控制腹侧中间神经元分化的分子和遗传机制方面已经取得了重大进展，但人们对它们沿脊髓前后轴的分布及其多样化知之甚少。在这里，我们报告 V0、V1 和 V2 中间神经元在发育中的脊髓的臂、胸和腰水平上表现出不同的组织模式。此外，我们证明，根据不同组转录因子的组合表达，每个主要类别的腹侧中间神经元可以细分为几个子集，并且这些子集沿着脊髓的头尾轴差异分布。这种腹侧中间神经元的全面分子分析为研究发育中脊髓的神经元多样化以及了解特定中间神经元子集对 CPG 回路和运动控制的贡献提供了重要资源。
The spinal cord contains neuronal circuits termed Central Pattern Generators (CPGs) that coordinate rhythmic motor activities. CPG circuits consist of motor neurons and multiple interneuron cell types, many of which are derived from four distinct cardinal classes of ventral interneurons, called V0, V1, V2 and V3. While significant progress has been made on elucidating the molecular and genetic mechanisms that control ventral interneuron differentiation, little is known about their distribution along the antero-posterior axis of the spinal cord and their diversification. Here, we report that V0, V1 and V2 interneurons exhibit distinct organizational patterns at brachial, thoracic and lumbar levels of the developing spinal cord. In addition, we demonstrate that each cardinal class of ventral interneurons can be subdivided into several subsets according to the combinatorial expression of different sets of transcription factors, and that these subsets are differentially distributed along the rostrocaudal axis of the spinal cord. This comprehensive molecular profiling of ventral interneurons provides an important resource for investigating neuronal diversification in the developing spinal cord and for understanding the contribution of specific interneuron subsets on CPG circuits and motor control.
登录
查看更多内容
影响因子：
2.7
作者：
Agius, E;Soukkarieh, C;Cochard, P
通讯作者：
Cochard, P
影响因子：
4.8
作者：
Boscher, Cecile;Mege, Rene-Marc
通讯作者：
Mege, Rene-Marc
影响因子：
64.5
作者：
Dasen, Jeremy S.;De Camilli, Alessandro;Jessell, Thomas M.
通讯作者：
Jessell, Thomas M.
影响因子：
5.5
作者：
Alvarez, FJ;Dewey, DE;Fyffe, REW
通讯作者：
Fyffe, REW
影响因子：
64.8
作者：
Dasen, JS;Liu, JP;Jessell, TM
通讯作者：
Jessell, TM