Clonal analysis of basophil differentiation in bone marrow cultures from a Down's syndrome patient with megakaryoblastic leukemia.
Clonal analysis of basophil differentiation in bone marrow cultures from a Down's syndrome patient with megakaryoblastic leukemia.
复制标题
患有巨核细胞白血病的唐氏综合症患者骨髓培养物中嗜碱性粒细胞分化的克隆分析。
作者：
T. Suda;J. Suda;Y. Miura;Yashuhide Hayashi;M. Eguchi;K. Tadokoro;M. Saito
我们介绍了在植物血凝素刺激的白细胞条件培养基中，使用液体培养和甲基纤维素培养系统，将唐氏综合征伴巨核母细胞白血病患者的骨髓细胞体外分化为嗜碱性细胞。通过甲苯胺蓝偏色染色、透射电镜和组胺的存在，建立了嗜碱性粒细胞的鉴定。然而，这些嗜碱性细胞在钙离子载体或趋化肽的作用下不释放组胺。含有90%嗜碱性粒细胞的悬浮培养样本在所有检查的有丝分裂中显示白血病细胞特征的染色体标记（48，XY, +11, +21, t(1;15)）。利用微操作技术对单细胞甲基纤维素培养白血病菌落的细胞组成进行了研究。观察到高电镀效率和极端优势的嗜碱性细胞菌落。在总共137个培养中，79个显示出菌落生长。细胞学检查分析的59个菌落中，46个为纯嗜碱性粒细胞菌落。这些嗜碱性细胞菌落呈分散形态，与正常的嗜碱性细胞菌落相似。从白血病单细胞培养中记录了嗜碱性粒细胞菌落的克隆性和谱系表达的倾斜。这些数据表明，白血病细胞具有分化成一些在体内不表达的谱系的能力。
We present the in vitro differentiation of marrow cells from a patient with Down's syndrome accompanied by megakaryoblastic leukemia into basophils in the presence of phytohemagglutinin-stimulated leukocyte conditioned medium, using a liquid culture and methylcellulose culture system. Identification of basophils was established by metachromatic staining with toluidine blue, transmission electron microscopy, and the presence of histamine. However, these basophils did not release histamine in response to calcium ionophore or chemotactic peptide. Samples from suspension cultures that contained 90% basophils showed chromosomal markers characteristic of leukemic cells (48, XY, +11, +21, t(1;15)) in all examined mitoses. The cellular composition of leukemic colonies grown in methylcellulose culture from single cells was studied using the micromanipulation technique. High plating efficiency and extreme predominance of basophil colonies were observed. In a total 137 cultures, 79 revealed colony growth. Of 59 colonies that were analyzed by cytologic examination, 46 were pure basophil colonies. These basophil colonies showed disperse morphology, similar to that of a normal basophil colony. The clonality of the basophil colonies and skewing of lineage expression were documented from leukemic single-cell cultures. These data showed that leukemic cells have the capacity for differentiation into some lineages that are not expressed in vivo.
DOI：
10.1172/jci111645
发表时间：
1984
期刊：
The Journal of clinical investigation
影响因子：
--
作者：
Leary,AG;Ogawa,M;Strauss,LC;Civin,CI
通讯作者：
Civin,CI