The rice GERMINATION DEFECTIVE 1, encoding a B3 domain transcriptional repressor, regulates seed germination and seedling development by integrating GA and carbohydrate metabolism.
The rice GERMINATION DEFECTIVE 1, encoding a B3 domain transcriptional repressor, regulates seed germination and seedling development by integrating GA and carbohydrate metabolism.
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水稻 GERMINATION DEFECTIVE 1 编码 B3 结构域转录阻遏蛋白，通过整合 GA 和碳水化合物代谢来调节种子萌发和幼苗发育
DOI：
10.1111/tpj.12209
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发表时间：
2013-08
期刊：
影响因子：
--
通讯作者：
Chu C
中科院分区：
文献类型：
--
作者：
Guo X;Hou X;Fang J;Wei P;Xu B;Chen M;Feng Y;Chu C
研究表明，拟南芥种子发育受转录因子网络的调节，包括 LEC1 (LEAFY COTYLEDON1)、L1L (LEC1-like) 和 B3 结构域因子 LEC2、FUS3 (FUSCA3) 和 ABI3 (ABA-INSENSITIVE3)；然而，人们对谷物种子发育的分子和遗传调控知之甚少。为了了解谷物的种子发育和种子萌发，我们使用我们的 T-DNA 突变体群体进行了大规模筛选，并鉴定了突变体萌发缺陷 1 (gd1)。除了严重的发芽缺陷外，gd1突变体还表现出矮化表型和异常的花发育。分子和生化分析表明，GD1 编码具有抑制活性的包含 B3 结构域的转录因子。与gd1的矮化表型一致，赤霉酸（GA）失活基因OsGA2ox3的表达显着增加，同时gd1中GA生物合成基因（包括OsGA20ox1、OsGA20ox2和OsGA3ox2）的表达减少，导致内源GA4水平降低。外源应用GA不仅诱导GD1表达，而且部分挽救了gd1的矮化表型。此外，GD1 通过 RY 元件与 OsLFL1（水稻的 LEC2/FUS3 样基因）的启动子结合，导致 OsLFL1 和 gd1 突变体中大部分种子成熟基因的显着上调。过表达 OsLFL1 的植物部分模仿了 gd1 突变体。此外，GD1的表达在糖处理下被诱导，并且gd1突变体中淀粉和可溶性糖的含量发生改变。这些数据表明GD1直接或间接参与调节GA和碳水化合物稳态，并进一步调节水稻种子萌发和幼苗发育。
It has been shown that seed development is regulated by a network of transcription factors in Arabidopsis including LEC1 (LEAFY COTYLEDON1), L1L (LEC1-like) and the B3 domain factors LEC2, FUS3 (FUSCA3) and ABI3 (ABA-INSENSITIVE3); however, molecular and genetic regulation of seed development in cereals is poorly understood. To understand seed development and seed germination in cereals, a large-scale screen was performed using our T–DNA mutant population, and a mutant germination-defective1 (gd1) was identified. In addition to the severe germination defect, the gd1 mutant also shows a dwarf phenotype and abnormal flower development. Molecular and biochemical analyses revealed that GD1 encodes a B3 domain-containing transcription factor with repression activity. Consistent with the dwarf phenotype of gd1, expression of the gibberelic acid (GA) inactivation gene OsGA2ox3 is increased dramatically, accompanied by reduced expression of GA biosynthetic genes including OsGA20ox1, OsGA20ox2 and OsGA3ox2 in gd1, resulting in a decreased endogenous GA4 level. Exogenous application of GA not only induced GD1 expression, but also partially rescued the dwarf phenotype of gd1. Furthermore, GD1 binds to the promoter of OsLFL1, a LEC2/FUS3-like gene of rice, via an RY element, leading to significant up-regulation of OsLFL1 and a large subset of seed maturation genes in the gd1 mutant. Plants over-expressing OsLFL1 partly mimic the gd1 mutant. In addition, expression of GD1 was induced under sugar treatment, and the contents of starch and soluble sugar are altered in the gd1 mutant. These data indicate that GD1 participates directly or indirectly in regulating GA and carbohydrate homeostasis, and further regulates rice seed germination and seedling development.
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影响因子：
7.4
作者：
Agrawal, GK;Yamazaki, M;Hirochika, H
通讯作者：
Hirochika, H
影响因子：
11.6
作者：
Li, Juan;Jiang, Jiafu;Chong, Kang
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Chong, Kang
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7.4
作者：
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11.6
作者：
Belin, Christophe;Megies, Christian;Lopez-Molina, Luis
通讯作者：
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