Mechanism of action of a novel recombinant peptide, MP1102, against Clostridium perfringens type C
Mechanism of action of a novel recombinant peptide, MP1102, against Clostridium perfringens type C
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新型重组肽 MP1102 对抗 C 型产气荚膜梭菌的作用机制
DOI：
10.1007/s00253-016-7387-x
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发表时间：
2016-02
期刊：
影响因子：
--
通讯作者：
Wang Jianhua
中科院分区：
文献类型：
--
作者：
Zong Lifen;Teng Da;Wang Xiumin;Mao Ruoyu;Yang Na;Hao Ya;Wang Jianhua
This work is the first to report the antibacterial characteristics and antibacterial mechanisms of MP1102, which is a variant of NZ2114, against pathogenic Clostridium perfringens. MP1102 exhibited strong antimicrobial activity against C. perfringens strains CVCC 61, CVCC 1163, and CVCC 2032 at a low minimal inhibitory concentration (MIC) of 0.91 μM. MP1102 showed anti-C. perfringens activity over a wide pH range of 2.0 and 10.0, high thermal stability from 20 to 80 °C, and remarkable resistance to pepsin. The fractional inhibitory concentration index (FICI) indicated an additive or synergic effect between MP1102 and bacitracin zinc, nisin, vancomycin, virginiamycin, aureomycin, and ampicillin against C. perfringens (FICI = 0.3125-1.0). To further elucidate the antibacterial mechanism of MP1102, its effect on the C. perfringens CVCC 61 cell membrane and intracellular DNA was studied. Flow cytometry and scanning electron microscopy (SEM) indicated that MP1102 treatment resulted in the release of cellular contents by damaging the membrane. A DNA gel retardation and circular dichroism analysis demonstrated that MP1102 interacted with DNA and intercalated into the DNA base pairs. A cell cycle assay demonstrated that MP1102 affected cellular functions, such as DNA synthesis. These results suggested that MP1102 exhibited potential as a new antimicrobial agent against C. perfringens infections.
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影响因子：
4.6
作者：
Tripathi JK;Kathuria M;Kumar A;Mitra K;Ghosh JK
通讯作者：
Ghosh JK
影响因子：
5
作者：
Yong Zhang;D. Teng;Xiumin Wang;R. Mao;Xintao Cao;Xiaoyuan Hu;Lifen Zong;Jianhua Wang
通讯作者：
Yong Zhang;D. Teng;Xiumin Wang;R. Mao;Xintao Cao;Xiaoyuan Hu;Lifen Zong;Jianhua Wang
影响因子：
64.8
作者：
Mygind, PH;Fischer, RL;Kristensen, HH
通讯作者：
Kristensen, HH
影响因子：
4.4
作者：
Gaucher, M-L.;Quessy, S.;Boulianne, M.
通讯作者：
Boulianne, M.
影响因子：
4.4
作者：
Jiao, Jian;Mao, Ruoyu;Wang, Jianhua
通讯作者：
Wang, Jianhua