Brief report: Mechanism of extravasation of infused stem cells.
Brief report: Mechanism of extravasation of infused stem cells.
复制标题
DOI：
10.1002/stem.1184
复制
发表时间：
2012-12
期刊：
影响因子：
5.2
通讯作者：
Marban, Eduardo
中科院分区：
文献类型：
--
作者：
Cheng, Ke;Shen, Deliang;Xie, Yucai;Cingolani, Eugenio;Malliaras, Konstantinos;Marban, Eduardo
为了使造血干细胞在组织再生中有效，细胞必须穿过血管壁并进入实质。虽然这种轮回确实发生了，但其机制仍然难以捉摸。白细胞通过渗出侵入组织;干细胞通常被认为也是如此，但缺乏证据。将心脏来源的再生细胞和多细胞心脏球注入大鼠心脏的冠状血管中。连续组织学显示了一种新的细胞迁移机制，“主动血管排出”，这是输注细胞和细胞聚集体外渗的基础。在这种机制中，血管屏障经历广泛的重塑，而细胞本身相对被动。通过在背部皮瓣模型中对心脏球外渗进行系列活体显微镜检查，证实了该机制。整合素和基质金属蛋白酶在主动血管排出中起关键作用。体外模型显示，主动血管排出可推广到其他干细胞类型和乳腺癌细胞。认识到主动血管排出作为经血管细胞迁移的机制为增强血管递送细胞治疗的功效开辟了新的机会。
In order for bloodborne stem cells to be effective in tissue regeneration, cells must cross vessel walls and enter the parenchyma. Although such transmigration does occur, the mechanism remains elusive. Leukocytes invade tissue by diapedesis; stem cells are commonly assumed to do likewise, but evidence is lacking. Cardiac-derived regenerative cells and multi-cellular cardiospheres were infused into the coronary vessels of rat hearts. Serial histology revealed a novel mechanism of cell transmigration, “active vascular expulsion”, which underlies the extravasation of infused cells and cell aggregates. In this mechanism, the vascular barrier undergoes extensive remodeling, while the cells themselves are relatively passive. The mechanism was confirmed in vivo by serial intravital microscopy of cardiosphere extravasation in a dorsal skin flap model. Integrins and matrix metalloproteinases play critical roles in active vascular expulsion. In vitro models reveal that active vascular expulsion is generalizable to other stem cell types and to breast cancer cells. Recognition of active vascular expulsion as a mechanism for transvascular cell migration opens new opportunities to enhance the efficacy of vascularly-delivered cell therapy.
登录
查看更多内容
影响因子：
168.9
作者：
Makkar, Raj R.;Smith, Rachel R.;Cheng, Ke;Malliaras, Konstantinos;Thomson, Louise E. J.;Berman, Daniel;Czer, Lawrence S. C.;Marban, Linda;Mendizabal, Adam;Johnston, Peter V.;Russell, Stuart D.;Schuleri, Karl H.;Lardo, Albert C.;Gerstenblith, Gary;Marban, Eduardo
通讯作者：
Marban, Eduardo
影响因子：
5.2
作者：
Li, Tao-Sheng;Cheng, Ke;Lee, Shuo-Tsan;Matsushita, Satoshi;Davis, Darryl;Malliaras, Konstantinos;Zhang, Yiqiang;Matsushita, Noriko;Smith, Rachel Ruckdeschel;Marban, Eduardo
通讯作者：
Marban, Eduardo
影响因子：
64.5
作者：
MAYADAS, TN;JOHNSON, RC;WAGNER, DD
通讯作者：
WAGNER, DD
影响因子：
5.2
作者：
Rampon, Christine;Weiss, Nicolas;Couraud, Pierre-Olivier
通讯作者：
Couraud, Pierre-Olivier
影响因子：
64.5
作者：
Somers, WS;Tang, J;Camphausen, RT
通讯作者：
Camphausen, RT