Cell-free DNA Release in the Plasma of Patients with Cardiac Disease is Associated with Cell Death Processes
Cell-free DNA Release in the Plasma of Patients with Cardiac Disease is Associated with Cell Death Processes
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心脏病患者血浆中游离 DNA 的释放与细胞死亡过程相关
DOI：
10.1007/s12291-022-01034-y
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发表时间：
2022
影响因子：
2.1
通讯作者：
Takeshita Haruo
中科院分区：
文献类型：
--
作者：
Fujihara Junko;Takinami Yoshikazu;Kimura-Kataoka Kaori;Kawai Yasuyuki;Takeshita Haruo
无细胞DNA（cfDNA）被释放到心脏病患者的血浆中。在此，研究了心肌梗死（MI）和其他心脏疾病患者（n= 59）中血浆cfDNA释放的来源和机制。测定各种标志物的血浆水平，包括M30（凋亡）、M65（凋亡和坏死）、亲环素A（CyPA）（坏死）和髓过氧化物酶（MPO）（中性粒细胞活化）。MI和其他心脏疾病患者的血浆cfDNA浓度显著高于健康对照受试者。在心脏病患者（MI和其他心脏病）和健康对照受试者中未观察到M30、M65和CyPA水平的显著差异。与此相反，MPO水平在心脏病患者中显着升高时，与对照组相比，心肌梗死患者的MPO水平显着高于其他心脏病患者。这些结果表明，cfDNA主要由中性粒细胞通过NETosis释放，除了心脏病患者中的上皮细胞凋亡之外，还通过细胞凋亡释放，并且在MI患者中程度更大。本研究结果为心肌梗死的诊断指标提供了基础信息。
Cell-free DNA (cfDNA) is released into the plasma of patients with cardiac disease. Here, the source and mechanism of plasma cfDNA release in patients with myocardial infarction (MI) and other cardiac diseases (n= 59) were investigated. Plasma levels of various markers including M30 (apoptosis), M65 (apoptosis and necrosis), cyclophilin A (CyPA) (necrosis), and myeloperoxidase (MPO) (neutrophil activation) were assayed. The plasma cfDNA concentrations in MI and other cardiac diseases were significantly higher than that in the healthy control subjects. Significant differences were not observed among the cardiac disease patients (MI and other cardiac diseases) and healthy control subjects in M30, M65, and CyPA levels. In contrast,the MPO levels were significantly elevated in cardiac disease patients when compared to control groups, and MPO levels in MI patients were significantly higher than other cardiac diseases patients. These results suggest that cfDNA is mainly released by neutrophils via NETosis in addition to apoptosis except for epithelial apoptosis in patients with cardiac disease and the degree is greater in MI patients. The results from this study provide basic information for diagnosis marker of MI.
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晚期非小细胞肺癌患者与对照组血清M30和M65值的比较
影响因子：
3.4
作者：
B. Oven Ustaalioglu;A. Bilici;Ş. Ercan;A. Orçun;M. Seker;A. Ozkan;Recep Ustaalioğlu;M. Gumus
通讯作者：
M. Gumus
影响因子：
4.3
作者：
S. Zheng;Shuang Liu;Mei Liu;M. McCrae;Jun-feng Li;Yuanping Han;Chun Xu;F. Ren;Yu Chen;Z. Duan
通讯作者：
S. Zheng;Shuang Liu;Mei Liu;M. McCrae;Jun-feng Li;Yuanping Han;Chun Xu;F. Ren;Yu Chen;Z. Duan
DOI：
10.5114/aoms.2015.56341
发表时间：
2015-12-10
期刊：
Archives of medical science : AMS
影响因子：
--
作者：
Pokrywka A;Zembron-Lacny A;Baldy-Chudzik K;Orysiak J;Sitkowski D;Banach M
通讯作者：
Banach M
影响因子：
3.5
作者：
Rudolph, Volker;Goldmann, Britta U.;Baldus, Stephan
通讯作者：
Baldus, Stephan
DOI：
10.1016/s0753-3322(05)80078-2
发表时间：
2005-10
期刊：
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie
影响因子：
--
作者：
T. Ueno;M. Toi;S. Linder
通讯作者：
T. Ueno;M. Toi;S. Linder