Dynamically expressed single ELAV/Hu orthologue elavl2 of bees is required for learning and memory.
Dynamically expressed single ELAV/Hu orthologue elavl2 of bees is required for learning and memory.
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DOI：
10.1038/s42003-021-02763-1
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发表时间：
2021-10-28
影响因子：
5.9
通讯作者：
Soller M
中科院分区：
文献类型：
--
作者：
Ustaoglu P;Gill JK;Doubovetzky N;Haussmann IU;Dix TC;Arnold R;Devaud JM;Soller M
基因表达的变化是学习和记忆巩固的标志。关于替代的信使核糖核酸加工，尤其是丰富的神经元特异性基因，如何在这些过程中发挥作用，我们知之甚少。神经元表达的ELAV/Hu家族的原型RNA结合蛋白是学习和记忆中的候选角色，但它们交叉调节和接管彼此功能的能力使这些联系的确证变得复杂。蜜蜂只有一个elav/Hu家族基因elavl2，通过增加包括进化保守的微外显子在内的选择性剪接，使其功能多样化。RNAi敲除表明ELAVL2在蜜蜂的学习和记忆中是必需的。ELAVL2在蘑菇体中通过改变选择性剪接和亚细胞定位而动态表达，但在其他脑区不表达。记忆巩固过程中elavl2的表达和选择性剪接的变化说明了作为记忆巩固基础的局部基因表达反应的一部分的替代mRNA处理程序。Ustaoglu，Gill等人。研究单拷贝的elav/Hu家族基因在蜜蜂学习和记忆中的作用。ELAVL2的RNAi敲除显示了在学习和记忆中的作用，并且ELAVL2通过改变选择性剪接和在学习时改变表达来动态表达。
Changes in gene expression are a hallmark of learning and memory consolidation. Little is known about how alternative mRNA processing, particularly abundant in neuron-specific genes, contributes to these processes. Prototype RNA binding proteins of the neuronally expressed ELAV/Hu family are candidates for roles in learning and memory, but their capacity to cross-regulate and take over each other’s functions complicate substantiation of such links. Honey bees Apis mellifera have only one elav/Hu family gene elavl2, that has functionally diversified by increasing alternative splicing including an evolutionary conserved microexon. RNAi knockdown demonstrates that ELAVL2 is required for learning and memory in bees. ELAVL2 is dynamically expressed with altered alternative splicing and subcellular localization in mushroom bodies, but not in other brain regions. Expression and alternative splicing of elavl2 change during memory consolidation illustrating an alternative mRNA processing program as part of a local gene expression response underlying memory consolidation. Ustaoglu, Gill, et al. investigate the role of the single copy of the elav/Hu family gene in honeybees in learning and memory. RNAi knockdown of elavl2 demonstrates a role in learning and memory, and ELAVL2 is dynamically expressed with altered alternative splicing and changing expression upon learning.
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影响因子：
4.6
作者：
Decio P;Ustaoglu P;Derecka K;Hardy ICW;Roat TC;Malaspina O;Mongan N;Stöger R;Soller M
通讯作者：
Soller M
影响因子：
12.3
作者：
Haussmann IU;White K;Soller M
通讯作者：
Soller M
DOI：
10.1007/978-1-4939-7737-6_12
发表时间：
2018-01-01
期刊：
EUKARYOTIC GENOMIC DATABASES: METHODS AND PROTOCOLS
影响因子：
--
作者：
Cary, Gregory A.;Cameron, R. Andrew;Hinman, Veronica F.
通讯作者：
Hinman, Veronica F.
影响因子：
3.6
作者：
Demares, Fabien;Drouard, Florian;Armengaud, Catherine
通讯作者：
Armengaud, Catherine
影响因子：
14.9
作者：
Drozdetskiy A;Cole C;Procter J;Barton GJ
通讯作者：
Barton GJ