Thiamethoxam exposure deregulates short ORF gene expression in the honey bee and compromises immune response to bacteria.
Thiamethoxam exposure deregulates short ORF gene expression in the honey bee and compromises immune response to bacteria.
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DOI：
10.1038/s41598-020-80620-7
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发表时间：
2021-01-15
影响因子：
4.6
通讯作者：
Soller M
中科院分区：
文献类型：
--
作者：
Decio P;Ustaoglu P;Derecka K;Hardy ICW;Roat TC;Malaspina O;Mongan N;Stöger R;Soller M
最大限度地提高作物产量依赖于使用农用化学品来控制害虫。最广泛使用的杀虫剂之一是类烟碱，它会干扰神经递质乙酰胆碱的信号传导，但也会破坏蜜蜂提供的作物授粉服务。在这里，我们分析了慢性低剂量长期暴露于类烟碱噻虫嗪是否会改变非洲蜜蜂（Apis mellifera）大脑中的基因表达和选择性剪接，以适应改变的神经元信号传导。我们发现差异调节的基因，显示浓度依赖性反应噻虫嗪，但没有变化的选择性剪接。大多数差异表达基因没有注释功能，但编码短开放阅读框，这是抗菌肽的特征。由于这表明免疫反应可能会受到噻虫嗪暴露的影响，我们通过注射细菌测试了噻虫嗪对蜜蜂免疫力的影响。我们表明，本质上亚致死噻虫嗪暴露使蜜蜂更容易受到正常的非致病性细菌。我们的研究结果意味着观察到的蜜蜂种群下降的协同机制，关注农业学家，保护生态学家和公众。
Maximizing crop yields relies on the use of agrochemicals to control insect pests. One of the most widely used classes of insecticides are neonicotinoids that interfere with signalling of the neurotransmitter acetylcholine, but these can also disrupt crop-pollination services provided by bees. Here, we analysed whether chronic low dose long-term exposure to the neonicotinoid thiamethoxam alters gene expression and alternative splicing in brains of Africanized honey bees, Apis mellifera, as adaptation to altered neuronal signalling. We find differentially regulated genes that show concentration-dependent responses to thiamethoxam, but no changes in alternative splicing. Most differentially expressed genes have no annotated function but encode short Open Reading Frames, a characteristic feature of anti-microbial peptides. As this suggested that immune responses may be compromised by thiamethoxam exposure, we tested the impact of thiamethoxam on bee immunity by injecting bacteria. We show that intrinsically sub-lethal thiamethoxam exposure makes bees more vulnerable to normally non-pathogenic bacteria. Our findings imply a synergistic mechanism for the observed bee population declines that concern agriculturists, conservation ecologists and the public.
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影响因子：
9.8
作者：
Dong, Yuemei;Taylor, Harry E;Dimopoulos, George
通讯作者：
Dimopoulos, George
影响因子：
3.7
作者：
De Smet L;Hatjina F;Ioannidis P;Hamamtzoglou A;Schoonvaere K;Francis F;Meeus I;Smagghe G;de Graaf DC
通讯作者：
de Graaf DC
影响因子：
12.3
作者：
Barribeau SM;Sadd BM;du Plessis L;Brown MJ;Buechel SD;Cappelle K;Carolan JC;Christiaens O;Colgan TJ;Erler S;Evans J;Helbing S;Karaus E;Lattorff HM;Marxer M;Meeus I;Näpflin K;Niu J;Schmid-Hempel R;Smagghe G;Waterhouse RM;Yu N;Zdobnov EM;Schmid-Hempel P
通讯作者：
Schmid-Hempel P
影响因子：
3.7
作者：
Dickel F;Münch D;Amdam GV;Mappes J;Freitak D
通讯作者：
Freitak D
影响因子：
2.7
作者：
Haussmann, Irmgard U.;Soller, Matthias
通讯作者：
Soller, Matthias