Complex representation of taste quality by second-order gustatory neurons in Drosophila.
Complex representation of taste quality by second-order gustatory neurons in Drosophila.
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DOI：
10.1016/j.cub.2022.07.048
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发表时间：
2022-09-12
期刊：
影响因子：
9.2
通讯作者：
Barnea, Gilad
中科院分区：
文献类型：
--
作者：
Snell, Nathaniel J.;Fisher, John D.;Hartmann, Griffin G.;Zolyomi, Bence;Talay, Mustafa;Barnea, Gilad
Sweet and bitter compounds excite different sensory cells and drive opposing behaviors. However, it remains unclear how sweet and bitter tastes are represented by the neural circuits linking sensation to behavior. To investigate this question in Drosophila, we devised trans-Tango(activity), a strategy for calcium imaging of second-order gustatory projection neurons based on trans-Tango, a genetic transsynaptic tracing technique. We found spatial overlap between the projection neuron populations activated by sweet and bitter tastants. The spatial representation of bitter tastants in the projection neurons was consistent, while that of sweet tastants was heterogeneous. Further, we discovered that bitter tastants evoke responses in the gustatory receptor neurons and projection neurons upon both stimulus onset and offset, and that bitter offset and sweet onset excite overlapping second-order projections. These findings demonstrate an unexpected complexity in the representation of sweet and bitter tastants by second-order neurons of the gustatory circuit. Snell et al. adapt a transsynaptic labeling strategy to image calcium responses of second-order gustatory projection neurons in Drosophila. Sweet and bitter activate overlapping populations of projection neurons. Projection neurons respond to both bitter onset and offset, and some regions respond to both sweet onset and bitter offset.
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