Complete mitochondrial and rDNA complex sequences of important vector species of Biomphalaria, obligatory hosts of the human-infecting blood fluke, Schistosoma mansoni.
Complete mitochondrial and rDNA complex sequences of important vector species of Biomphalaria, obligatory hosts of the human-infecting blood fluke, Schistosoma mansoni.
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DOI：
10.1038/s41598-018-25463-z
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发表时间：
2018-05-09
影响因子：
4.6
通讯作者：
Loker ES
中科院分区：
文献类型：
--
作者：
Zhang SM;Bu L;Laidemitt MR;Lu L;Mutuku MW;Mkoji GM;Loker ES
我们利用高通量的Illumina测序技术，测定了非洲双脐螺属（Biomphalaria）中三个淡水螺类群（菲氏双脐螺（B. pfeifferi）、苏丹双脐螺（B. sudanica）和漏斗双脐螺（B. choanomphala））以及源自新热带地区的两个光滑双脐螺（B. glabrata）实验室品系的线粒体基因组（线粒体基因组）和核核糖体DNA（rDNA）复合体的完整序列。双脐螺是人体肠道血吸虫病的主要病原体——曼氏血吸虫（Schistosoma mansoni）的专性传播媒介。我们的数据表明，非洲和新热带地区双脐螺的线粒体基因组高度保守。就rDNA而言，两个内转录间隔区（ITS1和ITS2）高度可变，而三个核糖体RNA基因（28S、5.8S和18S rRNA）则没有变化或变化非常有限。我们的分析显示，栖息于维多利亚湖的两个类群，即苏丹双脐螺和漏斗双脐螺，彼此之间的相似程度，相较于它们与菲氏双脐螺或光滑双脐螺的相似程度要高得多。这些新的序列信息可能有助于开发用于蜗牛鉴定的新标记，用于环境检测/监测目的，或用于追踪流行地区曼氏血吸虫的流行病学情况以及蜗牛与血吸虫的依存关系。它还为双脐螺系统学和命名法中尚未解决的问题提供了新的相关信息。
Using high throughput Illumina sequencing technology, we determined complete sequences for the mitochondrial genome (mitogenome) and nuclear ribosomal DNA (rDNA) complex for three African freshwater snail taxa within the genus Biomphalaria, B. pfeifferi, B. sudanica and B. choanomphala, and for two laboratory strains of B. glabrata originating from the Neotropics. Biomphalaria snails are obligate vectors of the blood fluke Schistosoma mansoni, a major etiologic agent of human intestinal schistosomiasis. Our data show that mitogenomes from African and Neotropical Biomphalaria are highly conserved. With respect to rDNA, the two internal transcribed spacers (ITS1 and 2) were found to be highly variable whereas the three ribosomal RNA genes (28S, 5.8S and 18S rRNA) exhibited no or very limited variation. Our analyses reveal that the two taxa inhabiting Lake Victoria, B. sudanica and B. choanomphala, are very similar to one another relative to the similarity either shows to B. pfeifferi or B. glabrata. This new sequence information may prove useful for developing new markers for snail identification, environmental detection/monitoring purposes or for tracking epidemiology and snail dependencies of S. mansoni in endemic areas. It also provides new information pertinent to still unresolved questions in Biomphalaria systematics and nomenclature.
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影响因子：
16.6
作者：
Adema CM;Hillier LW;Jones CS;Loker ES;Knight M;Minx P;Oliveira G;Raghavan N;Shedlock A;do Amaral LR;Arican-Goktas HD;Assis JG;Baba EH;Baron OL;Bayne CJ;Bickham-Wright U;Biggar KK;Blouin M;Bonning BC;Botka C;Bridger JM;Buckley KM;Buddenborg SK;Lima Caldeira R;Carleton J;Carvalho OS;Castillo MG;Chalmers IW;Christensens M;Clifton S;Cosseau C;Coustau C;Cripps RM;Cuesta-Astroz Y;Cummins SF;di Stephano L;Dinguirard N;Duval D;Emrich S;Feschotte C;Feyereisen R;FitzGerald P;Fronick C;Fulton L;Galinier R;Gava SG;Geusz M;Geyer KK;Giraldo-Calderón GI;de Souza Gomes M;Gordy MA;Gourbal B;Grunau C;Hanington PC;Hoffmann KF;Hughes D;Humphries J;Jackson DJ;Jannotti-Passos LK;de Jesus Jeremias W;Jobling S;Kamel B;Kapusta A;Kaur S;Koene JM;Kohn AB;Lawson D;Lawton SP;Liang D;Limpanont Y;Liu S;Lockyer AE;Lovato TL;Ludolf F;Magrini V;McManus DP;Medina M;Misra M;Mitta G;Mkoji GM;Montague MJ;Montelongo C;Moroz LL;Munoz-Torres MC;Niazi U;Noble LR;Oliveira FS;Pais FS;Papenfuss AT;Peace R;Pena JJ;Pila EA;Quelais T;Raney BJ;Rast JP;Rollinson D;Rosse IC;Rotgans B;Routledge EJ;Ryan KM;Scholte LLS;Storey KB;Swain M;Tennessen JA;Tomlinson C;Trujillo DL;Volpi EV;Walker AJ;Wang T;Wannaporn I;Warren WC;Wu XJ;Yoshino TP;Yusuf M;Zhang SM;Zhao M;Wilson RK
通讯作者：
Wilson RK
DOI：
10.1111/j.1096-3642.2000.tb00017.x
发表时间：
2000-07-01
影响因子：
2.8
作者：
Bandoni, SM;Mulvey, M;Loker, ES
通讯作者：
Loker, ES
腹足动物线粒体基因组排列的进化。
影响因子：
3.4
作者：
Grande, Cristina;Templado, Jose;Zardoya, Rafael
通讯作者：
Zardoya, Rafael
影响因子：
1.7
作者：
Bankevich, Anton;Nurk, Sergey;Pevzner, Pavel A.
通讯作者：
Pevzner, Pavel A.
影响因子：
10.7
作者：
Kumar, Sudhir;Stecher, Glen;Tamura, Koichiro
通讯作者：
Tamura, Koichiro