A large insert Thellungiella halophila BIBAC library for genomics and identification of stress tolerance genes
A large insert Thellungiella halophila BIBAC library for genomics and identification of stress tolerance genes
复制标题
用于基因组学和胁迫耐受基因鉴定的大型插入盐藻 BIBAC 文库
DOI：
10.1007/s11103-009-9553-3
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发表时间：
2009
影响因子：
5.1
通讯作者：
Li, Yin
中科院分区：
文献类型：
--
作者：
Xie, Qi*;Gao, Peng;Zhang, Huawei;Xia, Guixian;Wu, Yaorong;Zhang, Zhonghui;Bao, Hongyan;Deng, Zhiyong;Zhang, Yiyue;Guo, Huishan;Xie, Jiaying;Lai, Jianbin;Yang, Chengwei;Wang, Weiquan;Yang, Cuiping;Tang, Sanyuan;Li, Yin
盐芥（Thellungiella halophila）是拟南芥的一种耐盐近缘植物，是研究植物耐非生物胁迫的重要模式植物。构建了一个双元细菌人工染色体（BIBAC）文库，这是第一个具有植物转化能力的盐芥大插入片段DNA文库。将小盐芥部分酶切的核DNA连接到双元载体pBIBAC 2的BamHI位点上，构建了BIBAC文库。该文库由23，040个克隆组成，平均插入片段大小为75 kb，覆盖了4个Thellungiella halophila单倍体基因组。选择含有超过50 kb插入片段的BIBAC克隆，并转化到拟南芥中用于耐盐植物筛选。从200个株系的筛选中发现一个转基因株系比野生型植株更耐盐。结果表明，该文库中含有抗逆基因的候选基因，该方法适用于对逆境敏感植物的遗传转化。
Salt cress (Thellungiella halophila), a salt-tolerant relative of Arabidopsis, has turned to be an important model plant for studying abiotic stress tolerance. One binary bacterial artificial chromosome (BIBAC) library was constructed which represents the first plant-transformation-competent large-insert DNA library generated for Thellungiella halophila. The BIBAC library was constructed in BamHI site of binary vector pBIBAC2 by ligation of partial digested nuclear DNA of Thellungiella halophila. This library consists of 23,040 clones with an average insert size of 75 kb, and covers 4x Thellungiella halophila haploid genomes. BIBAC clones which contain inserts over 50 kb were selected and transformed into Arabidopsis for salt tolerant plant screening. One transgenic line was found to be more salt tolerant than wild type plants from the screen of 200 lines. It was demonstrated that the library contains candidates of stress tolerance genes and the approach is suitable for the transformation of stress susceptible plants for genetic improvement.
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影响因子：
56.9
作者：
BJ Staskawicz;FM Ausubel;BJ Baker;JG Ellis;JD Jones
通讯作者：
BJ Staskawicz;FM Ausubel;BJ Baker;JG Ellis;JD Jones
影响因子：
5.4
作者：
Feng, Jiuhuan;Vick, Brady A.;Jan, C. C.
通讯作者：
Jan, C. C.
影响因子：
6.2
作者：
Chen, An-Ping;Wang, Gui-Ling;Xia, Gui-Xian
通讯作者：
Xia, Gui-Xian
影响因子：
7.4
作者：
Koyama, ML;Levesley, A;Yeo, AR
通讯作者：
Yeo, AR
影响因子：
6.9
作者：
Gao, Feng;Gao, Qiang;Zhang, Juren
通讯作者：
Zhang, Juren