A panel of autoantibodies against multiple tumor-associated antigens in the immunodiagnosis of esophageal squamous cell cancer
A panel of autoantibodies against multiple tumor-associated antigens in the immunodiagnosis of esophageal squamous cell cancer
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一组针对多种肿瘤相关抗原的自身抗体在食管鳞状细胞癌免疫诊断中的应用
DOI：
10.1007/s00262-016-1886-6
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发表时间：
2016-08
期刊：
影响因子：
--
通讯作者：
Zhang JY
中科院分区：
文献类型：
--
作者：
Zhang HF;Qin JJ;Ren PF;Shi JX;Xia JF;Ye H;Wang P;Song CH;Wang KJ;Zhang JY
食管鳞状细胞癌（ESCC）是我国常见的恶性肿瘤之一，由于缺乏有效的早期诊断方法，其5年生存率很低。采用酶联免疫吸附试验（ELISA）检测ESCC患者和健康对照者血清中9种肿瘤相关抗原（TAAs）的自身抗体，以评价其在ESCC免疫诊断中的作用。生成逻辑回归模型来预测训练队列（648名参与者）中个体被诊断患有ESCC的概率，并在另一个独立队列（372名参与者）中进一步验证。最后，一组四个TAA显示出较高的诊断准确性，接受者工作特征曲线下面积在训练队列中分别为0.838，在验证队列中分别为0.872。训练组和验证组的正确分类率分别为77.01%和78.49%，而训练组和验证组的正确分类率分别为77.01%和78.49%.该模型可区分早期（AJCC 0、I和II期）ESCC患者和正常对照，训练队列的真阳性率（TPR）为67.57%，验证队列的TPR为63.33%，当两个队列合并时，早期ESCC的总TPR为66.85%。该模型的诊断性能显示早期和晚期（AJCC III期和IV期）ESCC患者之间无显著差异。总之，具有4个TAA的优化模型对于ESCC检测具有高诊断性能，特别是对于早期ESCC。
Esophageal squamous cell carcinoma (ESCC) is one of the most common cancers in China with very low 5-year survival rate mostly due to the paucity of effective early diagnostic methods. Serum autoantibodies against 9 tumor-associated antigens (TAAs) from ESCC patients and healthy controls were detected by enzyme-linked immunosorbent assay to evaluate their performances in the immunodiagnosis of ESCC. Logistic regression models were generated to predict the probability of individuals being diagnosed with ESCC in training cohort (648 participants) and further validated in another independent cohort (372 participants). Finally, a panel of four TAAs showed high diagnostic accuracy with areas under the receiver operating characteristic curve of 0.838 in training cohort and 0.872 in validation cohort, respectively. The percentages of individuals correctly classified were 77.01 % in training cohort and 78.49 % in validation cohort, respectively. This model could discriminate early-stage (AJCC stage 0, I and II) ESCC patients from normal controls, with true-positive rate (TPR) of 67.57 % in training cohort and TPR of 63.33 % in validation cohort, and the overall TPR for early-stage ESCC was 66.85 % when the two cohorts were combined. The diagnostic performance of this model showed no significant difference between early-stage and late-stage (AJCC stage III and IV) ESCC patients. In summary, the optimized model with 4 TAAs has a high diagnostic performance for ESCC detection, especially for early-stage ESCC.
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影响因子：
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作者：
Huang ZL;Lin ZR;Xiao YR;Cao X;Zhu LC;Zeng MS;Zhong Q;Wen ZS
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DOI：
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发表时间：
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期刊：
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影响因子：
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作者：
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影响因子：
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作者：
Zhang, Chunni;Wang, Cheng;Zhang, Chen-Yu
通讯作者：
Zhang, Chen-Yu
DOI：
10.14343/jcscr.2016.4e1003
发表时间：
2016
期刊：
--
影响因子：
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作者：
M. Štimpfel;I. Virant-Klun
通讯作者：
M. Štimpfel;I. Virant-Klun