Calorie restriction effects on silencing and recombination at the yeast rDNA.
Calorie restriction effects on silencing and recombination at the yeast rDNA.
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DOI：
10.1111/j.1474-9726.2009.00516.x
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发表时间：
2009-12
期刊：
影响因子：
7.8
通讯作者：
Smith JS
中科院分区：
文献类型：
--
作者：
Smith DL Jr;Li C;Matecic M;Maqani N;Bryk M;Smith JS
衰老研究在过去十年中发展迅速，通过使用模式生物和高通量技术来识别衰老过程的个体基因和分子机制。热量限制（CR）是最广泛研究的延长寿命的环境操纵。NAD+依赖性蛋白质脱乙酰酶Sir2（沉默信息调节因子2）的激活已被提出介导CR在芽殖酵母S.酿酒酵母，以及其他生物。在这里，我们表明，与以前的报道相反，Sir2不受CR刺激，以加强S rDNA中多个报告基因的沉默。啤酒。CR确实适度地降低rDNA重组的频率，尽管是以不依赖于SIR2的方式。CR介导的rDNA重组的抑制也不与来自rDNA基因间间隔区的Pol II转录的非编码RNA的沉默相关，这表明另外的沉默独立途径在寿命调节中起作用。
Aging research has developed rapidly over the past decade, identifying individual genes and molecular mechanisms of the aging process through the use of model organisms and high throughput technologies. Calorie Restriction (CR) is the most widely researched environmental manipulation that extends lifespan. Activation of the NAD+-dependent protein deacetylase Sir2 (Silent Information Regulator 2) has been proposed to mediate the beneficial effects of CR in the budding yeast S. cerevisiae, as well as other organisms. Here we show that in contrast to previous reports, Sir2 is not stimulated by CR to strengthen silencing of multiple reporter genes in the rDNA of S. cerevisiae. CR does modestly reduce the frequency of rDNA recombination, although in a SIR2-independent manner. CR-mediated repression of rDNA recombination also does not correlate with the silencing of Pol II-transcribed non-coding RNAs derived from the rDNA intergenic spacer, suggesting that additional silencing-independent pathways function in lifespan regulation.
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