Identification of antimony- and arsenic-oxidizing bacteria associated with antimony mine tailing.
Identification of antimony- and arsenic-oxidizing bacteria associated with antimony mine tailing.
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DOI：
10.1264/jsme2.me12217
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发表时间：
2013
影响因子：
2.2
通讯作者：
Itai T
中科院分区：
文献类型：
--
作者：
Hamamura N;Fukushima K;Itai T
锑（Sb）是一种天然存在的有毒元素，通常与环境中的砷（As）有关，两种元素具有相似的化学性质和毒性。越来越多的研究集中在微生物的砷转化，而微生物的锑相互作用仍然没有得到很好的理解。为了深入了解锑和砷的地球化学循环中的微生物作用，从锑尾矿土壤中的Sb-和As-氧化细菌的存在进行了检查。在用AsIII（10 mM）或SbIII（100 μM）进行有氧富集培养后，获得了具有SbIII氧化活性的假单胞菌和寡养单胞菌相关分离物的纯培养物以及能够氧化AsIII的中华根瘤菌相关分离物。AsIII-氧化中华根瘤菌分离物具有好氧亚砷酸盐氧化酶基因（aioA），其表达在AsIII或Sb III的存在下诱导。然而，没有SbIII氧化活性检测中华根瘤菌相关的分离物，这表明参与不同的机制，锑和砷氧化。这些结果表明，土著微生物与锑矿山土壤是能够锑和砷的氧化，并可能有助于锑和砷的形态和流动性原位。
Antimony (Sb) is a naturally occurring toxic element commonly associated with arsenic (As) in the environment and both elements have similar chemistry and toxicity. Increasing numbers of studies have focused on microbial As transformations, while microbial Sb interactions are still not well understood. To gain insight into microbial roles in the geochemical cycling of Sb and As, soils from Sb mine tailing were examined for the presence of Sb- and As-oxidizing bacteria. After aerobic enrichment culturing with AsIII (10 mM) or SbIII (100 μM), pure cultures of Pseudomonas- and Stenotrophomonas-related isolates with SbIII oxidation activities and a Sinorhizobium-related isolate capable of AsIII oxidation were obtained. The AsIII-oxidizing Sinorhizobium isolate possessed the aerobic arsenite oxidase gene (aioA), the expression of which was induced in the presence of AsIII or SbIII. However, no SbIII oxidation activity was detected from the Sinorhizobium-related isolate, suggesting the involvement of different mechanisms for Sb and As oxidation. These results demonstrate that indigenous microorganisms associated with Sb mine soils are capable of Sb and As oxidation, and potentially contribute to the speciation and mobility of Sb and As in situ.
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影响因子：
11.4
作者：
Mitsunobu, Satoshi;Harada, Teppei;Takahashi, Yoshio
通讯作者：
Takahashi, Yoshio
影响因子：
2.3
作者：
Drewniak, Lukasz;Matlakowska, Renata;Sklodowska, Aleksandra
通讯作者：
Sklodowska, Aleksandra
影响因子：
11.4
作者：
Macur, RE;Jackson, CR;Inskeep, WP
通讯作者：
Inskeep, WP
影响因子：
3.5
作者：
Fu HL;Rosen BP;Bhattacharjee H
通讯作者：
Bhattacharjee H
不同砷污染土壤砷转化和抗性相关基因
影响因子：
4.2
作者：
Cai, Lin;Liu, Guanghui;Rensing, Christopher;Wang, Gejiao
通讯作者：
Wang, Gejiao