Emerging nanotechnology-based strategies for the identification of microbial pathogenesis.
Emerging nanotechnology-based strategies for the identification of microbial pathogenesis.
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DOI：
10.1016/j.addr.2009.11.013
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发表时间：
2010-03-18
影响因子：
16.1
通讯作者：
Perez, J. Manuel
中科院分区：
文献类型：
--
作者：
Kaittanis, Charalambos;Santra, Santimukul;Perez, J. Manuel
关键词：
传染病仍然是一个主要的卫生保健问题。从食物中毒和受污染的水，到医院获得性疾病和流行病，传染性病原体在世界各地引起疾病。尽管病原体鉴定取得了进展，但一些金标准诊断方法存在局限性，包括费力的样品制备，笨重的仪器和缓慢的数据读取。此外，在第一反应者和护理点应用中迫切需要新的可现场部署的诊断模式。除了紧凑之外，这些传感器还必须灵敏、特异、坚固和快速，以便即使在偏远的农村地区也能方便地检测病原体。考虑到这些特性，研究人员利用纳米材料的独特特性，利用创新方法来实现对感染因子的检测，即使是在血液等复杂介质中。从金纳米颗粒及其等离子体转变到氧化铁纳米颗粒和磁性变化，病原体，毒素，抗原和核酸的检测已经实现了令人印象深刻的检测阈值。此外，随着细菌对抗生素产生耐药性，纳米技术已经实现了使用新方法快速测定细菌药物敏感性和耐药性，例如电流分析法和磁弛豫法。总的来说，这些有希望的结果表明，在地球仪的不同环境中，采用基于纳米技术的诊断方法来诊断传染病，预防流行病，保障人类和经济健康。
Infectious diseases are still a major healthcare problem. From food intoxication and contaminated water, to hospital-acquired diseases and pandemics, infectious agents cause disease throughout the world. Despite advancements in pathogens’ identification, some of the gold-standard diagnostic methods have limitations, including laborious sample preparation, bulky instrumentation and slow data readout. In addition, new field-deployable diagnostic modalities are urgently needed in first responder and point of care applications. Apart from compact, these sensors must be sensitive, specific, robust and fast, in order to facilitate detection of the pathogen even in remote rural areas. Considering these characteristics, researchers have utilized innovative approaches by employing the unique properties of nanomaterials in order to achieve detection of infectious agents, even in complex media like blood. From gold nanoparticles and their plasmonic shifts to iron oxide nanoparticles and changes in magnetic properties, detection of pathogens, toxins, antigens and nucleic acids has been achieved with impressive detection thresholds. Additionally, as bacteria become resistant to antibiotics, nanotechnology has achieved the rapid determination of bacterial drug susceptibility and resistance using novel methods, such as amperometry and magnetic relaxation. Overall, these promising results hint to the adoption of nanotechnology-based diagnostics for the diagnosis of infectious diseases in diverse settings throughout the globe, preventing epidemics and safeguarding human and economic wellness.
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DOI：
10.4024/1050501.jbpc.05.01
发表时间：
2005-01-01
期刊：
Journal of Biological Physics and Chemistry
影响因子：
--
作者：
Dobozi-King, M.;Seo, S.;Kish, L. B.
通讯作者：
Kish, L. B.
影响因子：
10.8
作者：
Fornara, Andrea;Johansson, Petter;Johansson, Christer
通讯作者：
Johansson, Christer
影响因子：
64.8
作者：
Chait, Remy;Craney, Allison;Kishony, Roy
通讯作者：
Kishony, Roy
影响因子：
2.9
作者：
Carol, J;Gorseling, MCJK;Irth, H
通讯作者：
Irth, H
影响因子：
56.9
作者：
Bruchez, M;Moronne, M;Alivisatos, AP
通讯作者：
Alivisatos, AP