Mammalian and Invertebrate Models as Complementary Tools for Gaining Mechanistic Insight on Muscle Responses to Spaceflight.
Mammalian and Invertebrate Models as Complementary Tools for Gaining Mechanistic Insight on Muscle Responses to Spaceflight.
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DOI：
10.3390/ijms22179470
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发表时间：
2021-08-31
影响因子：
5.6
通讯作者：
Tahimic CGT
中科院分区：
文献类型：
--
作者：
Cahill T;Cope H;Bass JJ;Overbey EG;Gilbert R;da Silveira WA;Paul AM;Mishra T;Herranz R;Reinsch SS;Costes SV;Hardiman G;Szewczyk NJ;Tahimic CGT
生物信息学方法已证明有助于了解航天飞行的生物反应。航天实验仍然是资源密集型的，而且很少。一个突出的问题是如何最大限度地提高科学产出的数量有限的组学数据集从传统的动物模型，包括线虫，果蝇和啮齿动物。无脊椎动物模型的组学数据在预测哺乳动物对空间飞行的反应方面的效用尚未得到充分探讨。因此，我们对经历37天太空飞行的小鼠比目鱼肌和趾长伸肌（EDL）的转录组进行了比较分析。结果表明共享的压力反应和改变昼夜节律。EDL显示出更强的生长信号和Pde2a下调，可能是其对萎缩的抵抗力相对于比目鱼肌。太空飞行和后肢卸载小鼠的增殖，昼夜节律和神经元信号传导的差异调节。卧床休息的人类和太空飞行的啮齿动物肌肉中的共同基因调控表明了减轻太空和地球上肌肉萎缩的目标。太空飞行对C. elegans与EDL更相似。离散生活阶段的D.黑腹肌在预测EDL和比目鱼肌反应方面具有独特的效用。总之，航天导致肌肉类型和无脊椎动物模型之间的共享和离散的分子反应，可能会增加从啮齿动物航天和地面研究中获得的机制知识。
Bioinformatics approaches have proven useful in understanding biological responses to spaceflight. Spaceflight experiments remain resource intensive and rare. One outstanding issue is how to maximize scientific output from a limited number of omics datasets from traditional animal models including nematodes, fruitfly, and rodents. The utility of omics data from invertebrate models in anticipating mammalian responses to spaceflight has not been fully explored. Hence, we performed comparative analyses of transcriptomes of soleus and extensor digitorum longus (EDL) in mice that underwent 37 days of spaceflight. Results indicate shared stress responses and altered circadian rhythm. EDL showed more robust growth signals and Pde2a downregulation, possibly underlying its resistance to atrophy versus soleus. Spaceflight and hindlimb unloading mice shared differential regulation of proliferation, circadian, and neuronal signaling. Shared gene regulation in muscles of humans on bedrest and space flown rodents suggest targets for mitigating muscle atrophy in space and on Earth. Spaceflight responses of C. elegans were more similar to EDL. Discrete life stages of D. melanogaster have distinct utility in anticipating EDL and soleus responses. In summary, spaceflight leads to shared and discrete molecular responses between muscle types and invertebrate models may augment mechanistic knowledge gained from rodent spaceflight and ground-based studies.
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影响因子：
14.9
作者：
Cheon S;Park N;Cho S;Kim K
通讯作者：
Kim K
DOI：
10.1073/pnas.1419045111
发表时间：
2014-11-25
影响因子：
11.1
作者：
Akay, Turgay;Tourtellotte, Warren G.;Jessell, Thomas M.
通讯作者：
Jessell, Thomas M.
影响因子：
4.8
作者：
Annayev, Yunus;Adar, Sheera;Ye, Rui
通讯作者：
Ye, Rui
DOI：
10.1111/j.2517-6161.1995.tb02031.x
发表时间：
1995-01-01
影响因子：
5.8
作者：
BENJAMINI, Y;HOCHBERG, Y
通讯作者：
HOCHBERG, Y
DOI：
10.1152/ajpendo.00590.2009
发表时间：
2010-11-01
影响因子：
5.1
作者：
Alibegovic, A. C.;Sonne, M. P.;Vaag, A.
通讯作者：
Vaag, A.