Analysis of novel human papillomavirus type 16 late mRNAs in differentiated W12 cervical epithelial cells.
Analysis of novel human papillomavirus type 16 late mRNAs in differentiated W12 cervical epithelial cells.
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DOI：
10.1016/j.virol.2006.10.012
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发表时间：
2007-03-30
期刊：
影响因子：
3.7
通讯作者：
Graham, Sheila V.
中科院分区：
文献类型：
--
作者：
Milligan, Steven G.;Veerapraditsin, Thanapom;Ahamet, Boolang;Mole, Sarah;Graham, Sheila V.
关键词：
人乳头瘤病毒16型（HPV 16）的生命周期与其感染的上皮细胞的分化密切相关，并且生命周期中的晚期事件仅限于基底上层。在这里，我们使用从表达病毒晚期蛋白的分化的W12细胞（含有HPV 16基因组附加体拷贝的宫颈上皮细胞）分离的多腺苷酸化RNA的5′RACE来绘制病毒晚期mRNA。确定了13种不同的抄本。广泛的选择性剪接和使用两个晚期多聚腺苷酸化位点。在长调控区发现了一个新的启动子，与P97和Plate一样。E4和E5开放阅读框中的启动子是有活性的，产生转录物，其中L1或L2分别是第一个开放阅读框。最后，鉴定了可能编码新蛋白E6*^*E7、E6*^E4、E1 *^E4和E1 *^E2C（推定的阻遏物E2）的mRNA，表明HPV 16可能编码比先前接受的更多的晚期蛋白。
The life cycle of human papillomavirus type 16 (HPV16) is intimately linked to differentiation of the epithelium it infects, and late events in the life cycle are restricted to the suprabasal layers. Here we have used 5′RACE of polyadenylated RNA isolated from differentiated W12 cells (cervical epithelial cells containing episomal copies of the HPV16 genome) that express virus late proteins to map virus late mRNAs. Thirteen different transcripts were identified. Extensive alternative splicing and use of two late polyadenylation sites were noted. A novel promoter located in the long control region was detected as well as P97 and Plate. Promoters in the E4 and E5 open reading frames were active yielding transcripts where L1 or L2 respectively are the first open reading frames. Finally, mRNAs that could encode novel proteins E6*^*E7, E6*^E4, E1^*E4 and E1^E2C (putative repressor E2) were identified, indicating that HPV16 may encode more late proteins than previously accepted.
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DOI：
10.1083/jcb.115.4.887
发表时间：
1991-11
期刊：
The Journal of cell biology
影响因子：
--
作者：
Kozak M
通讯作者：
Kozak M
影响因子：
5.4
作者：
CRUM, CP;NUOVO, G;SILVERSTEIN, SJ
通讯作者：
SILVERSTEIN, SJ
影响因子：
5.4
作者：
HUMMEL, M;LIM, HB;LAIMINS, LA
通讯作者：
LAIMINS, LA
影响因子：
5.4
作者：
HUMMEL, M;HUDSON, JB;LAIMINS, LA
通讯作者：
LAIMINS, LA
影响因子：
5.4
作者：
Frattini, MG;Lim, HB;Laimins, LA
通讯作者：
Laimins, LA