Transcriptional profiling of human endogenous retrovirus group HERV-K(HML-2) loci in melanoma.
Transcriptional profiling of human endogenous retrovirus group HERV-K(HML-2) loci in melanoma.
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DOI：
10.1093/gbe/evt010
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发表时间：
2013
影响因子：
3.3
通讯作者：
Mayer J
中科院分区：
文献类型：
--
作者：
Schmitt K;Reichrath J;Roesch A;Meese E;Mayer J
最近的研究表明，人类内源性逆转录病毒（HERV）组HERV-K（HML-2）在黑色素瘤中的作用，因为HERV-K（HML-2）编码的蛋白质的转录和表达上调。很少有人知道哪些HML-2基因座在黑色素瘤中转录。我们将从各种黑色素瘤和相关样品产生的> 1，400个HML-2 cDNA序列分配到基因组HML-2基因座，鉴定出总共23个基因座转录。基因座的转录谱在样品之间差异显著。一个基因座被发现只在黑色素瘤衍生的样本中转录，但在黑色素细胞中不转录，可能代表黑色素瘤的标志物。几个转录的基因座含有逆转录病毒Gag和/或Env蛋白的ORF。Env编码位点仅在黑色素瘤中转录。rec和np 9转录本的特定研究表明黑色素瘤和黑色素细胞中蛋白质编码位点的转录，暗示了Rec和Np 9在黑色素瘤中的相关性。UVB照射改变了基因座的转录谱，黑色素瘤和黑素细胞的总体转录水平下降。我们进一步确定了转录HML-2基因座形成的剪接HML-2转录本的逆转录L1机制或在逆转录病毒的方式，与基因座可能编码HML-2样蛋白。我们揭示了黑色素瘤和相关样本中HML-2基因座复杂的样本特异性转录。鉴定的HML-2基因座和由这些基因座编码的蛋白对于进一步研究HML-2在黑素瘤中的作用特别相关。HERV的转录似乎是一种复杂的机制，需要进行具体研究来阐明哪些HERV基因座被转录以及转录的HERV如何参与疾病。
Recent studies suggested a role for the human endogenous retrovirus (HERV) group HERV-K(HML-2) in melanoma because of upregulated transcription and expression of HERV-K(HML-2)-encoded proteins. Very little is known about which HML-2 loci are transcribed in melanoma. We assigned >1,400 HML-2 cDNA sequences generated from various melanoma and related samples to genomic HML-2 loci, identifying a total of 23 loci as transcribed. Transcription profiles of loci differed significantly between samples. One locus was found transcribed only in melanoma-derived samples but not in melanocytes and might represent a marker for melanoma. Several of the transcribed loci harbor ORFs for retroviral Gag and/or Env proteins. Env-encoding loci were transcribed only in melanoma. Specific investigation of rec and np9 transcripts indicated transcription of protein encoding loci in melanoma and melanocytes hinting at the relevance of Rec and Np9 in melanoma. UVB irradiation changed transcription profiles of loci and overall transcript levels decreased in melanoma and melanocytes. We further identified transcribed HML-2 loci formed by reverse transcription of spliced HML-2 transcripts by L1 machinery or in a retroviral fashion, with loci potentially encoding HML-2-like proteins. We reveal complex, sample-specific transcription of HML-2 loci in melanoma and related samples. Identified HML-2 loci and proteins encoded by those loci are particularly relevant for further studying the role of HML-2 in melanoma. Transcription of HERVs appears as a complex mechanism requiring specific studies to elucidate which HERV loci are transcribed and how transcribed HERVs may be involved in disease.
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影响因子：
8
作者：
Boese, A;Sauter, M;Mueller-Lantzsch, N
通讯作者：
Mueller-Lantzsch, N
DOI：
10.1073/pnas.0307885100
发表时间：
2004-02-10
影响因子：
11.1
作者：
Hughes, JF;Coffin, JM
通讯作者：
Coffin, JM
影响因子：
5.4
作者：
Buzdin, Anton;Kovalskaya-Alexandrova, Elena;Sverdlov, Eugene
通讯作者：
Sverdlov, Eugene
影响因子：
5.4
作者：
Frank, O;Giehl, M;Seifarth, W
通讯作者：
Seifarth, W
影响因子：
9.2
作者：
Barbulescu, M;Turner, G;Lenz, J
通讯作者：
Lenz, J