Effect of water content on the glass transition temperature of mixtures of sugars, polymers, and penetrating cryoprotectants in physiological buffer.
Effect of water content on the glass transition temperature of mixtures of sugars, polymers, and penetrating cryoprotectants in physiological buffer.
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DOI：
10.1371/journal.pone.0190713
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发表时间：
2018
期刊：
影响因子：
3.7
通讯作者：
Higgins AZ
中科院分区：
文献类型：
--
作者：
Drake AC;Lee Y;Burgess EM;Karlsson JOM;Eroglu A;Higgins AZ
活哺乳动物细胞的长期储存对于从体外受精到细胞治疗的应用范围是重要的。冷冻保存是目前最常用的方法，但在液氮中储存相对昂贵，并且在运输过程中对低温的要求不方便。干燥是一种替代策略，有可能在更方便的储存温度下保存活细胞，而无需液氮。为了在干燥状态下储存期间实现稳定性，必须除去足够的水，使得剩余的基质形成非结晶玻璃状固体。因此，玻璃化转变温度是设计细胞干燥程序的关键参数。在这项研究中，我们研究了水分含量对糖（海藻糖或棉子糖），聚合物（聚乙烯吡咯烷酮或Ficoll），渗透性冷冻保护剂（乙二醇，丙二醇或二甲亚砜）和磷酸盐缓冲盐水（PBS）溶质的混合物的玻璃化转变温度（Tg）的影响。通过用饱和盐溶液平衡或通过在95°C下烘烤将水溶液干燥至不同的水分含量。然后通过差示扫描量热法测量脱水样品的玻璃化转变温度。正如预期的那样，Tg随着水分含量的降低而增加。例如，在PBS中含有0.1 M海藻糖的干燥介质中，Tg范围从完全干燥样品的约360 K到水质量分数为0.4时的约220 K。向溶液中添加聚合物增加了Tg，而添加渗透性冷冻保护剂降低了Tg。我们的研究结果提供了深入了解相对湿度，水分含量和玻璃化转变温度之间的关系，细胞干燥溶液中含有糖，聚合物和渗透冷冻保护剂。
Long-term storage of viable mammalian cells is important for applications ranging from in vitro fertilization to cell therapy. Cryopreservation is currently the most common approach, but storage in liquid nitrogen is relatively costly and the requirement for low temperatures during shipping is inconvenient. Desiccation is an alternative strategy with the potential to enable viable cell preservation at more convenient storage temperatures without the need for liquid nitrogen. To achieve stability during storage in the dried state it is necessary to remove enough water that the remaining matrix forms a non-crystalline glassy solid. Thus, the glass transition temperature is a key parameter for design of cell desiccation procedures. In this study, we have investigated the effects of moisture content on the glass transition temperature (Tg) of mixtures of sugars (trehalose or raffinose), polymers (polyvinylpyrrolidone or Ficoll), penetrating cryoprotectants (ethylene glycol, propylene glycol, or dimethyl sulfoxide), and phosphate buffered saline (PBS) solutes. Aqueous solutions were dried to different moisture contents by equilibration with saturated salt solutions, or by baking at 95°C. The glass transition temperatures of the dehydrated samples were then measured by differential scanning calorimetry. As expected, Tg increased with decreasing moisture content. For example, in a desiccation medium containing 0.1 M trehalose in PBS, Tg ranged from about 360 K for a completely dry sample to about 220 K at a water mass fraction of 0.4. Addition of polymers to the solutions increased Tg, while addition of penetrating cryoprotectants decreased Tg. Our results provide insight into the relationship between relative humidity, moisture content and glass transition temperature for cell desiccation solutions containing sugars, polymers and penetrating cryoprotectants.
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影响因子：
3.7
作者：
Abazari, Alireza;Meimetis, Labros G.;Toner, Mehmet
通讯作者：
Toner, Mehmet
DOI：
10.1002/jctb.5010020901
发表时间：
1952-01-01
期刊：
JOURNAL OF APPLIED CHEMISTRY
影响因子：
--
作者：
GORDON, M;TAYLOR, JS
通讯作者：
TAYLOR, JS
影响因子：
8.8
作者：
Lammert, AM;Schmidt, SJ;Day, GA
通讯作者：
Day, GA
影响因子：
13.3
作者：
Argyle, Catrin E.;Harper, Joyce C.;Davies, Melanie C.
通讯作者：
Davies, Melanie C.
影响因子：
3.4
作者：
Crowe, LM;Reid, DS;Crowe, JH
通讯作者：
Crowe, JH