Prototype nerve-specific near-infrared fluorophores.
Prototype nerve-specific near-infrared fluorophores.
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作者：
Park MH;Hyun H;Ashitate Y;Wada H;Park G;Lee JH;Njiojob C;Henary M;Frangioni JV;Choi HS
在大多数手术中，神经保护是一个重要的问题，因为意外的横断或损伤会导致显著的并发症，包括麻木、疼痛、虚弱或瘫痪。目前，神经的识别仍然只是根据手术中的大体外观和解剖位置，而没有术中的图像指导。近红外(NIR)荧光灯在650-900 nm的波长范围内，具有提供高分辨率、高灵敏度和实时避免神经损伤的潜力，但前提是可以开发出神经特有的近红外荧光团。在这项研究中，我们评估了一系列恶嗪衍生物以突出小动物和大动物的各种周围神经结构。在靶向荧光团中，恶嗪4具有近红外发射峰，在单次静脉注射后，可在臂丛和坐骨神经中提供长达12小时的神经靶向信号。此外，成功地识别并实时突出了猪的喉返神经，在甲状腺切除过程中可以保留这些神经。虽然这些试剂的光学性质还不是最理想的，但化学结构分析为进一步改进这些神经特异性的原型近红外荧光团提供了基础。
Nerve preservation is an important issue during most surgery because accidental transection or injury results in significant morbidity, including numbness, pain, weakness, or paralysis. Currently, nerves are still identified only by gross appearance and anatomical location during surgery, without intraoperative image guidance. Near-infrared (NIR) fluorescent light, in the wavelength range of 650-900 nm, has the potential to provide high-resolution, high-sensitivity, and real-time avoidance of nerve damage, but only if nerve-specific NIR fluorophores can be developed. In this study, we evaluated a series of Oxazine derivatives to highlight various peripheral nerve structures in small and large animals. Among the targeted fluorophores, Oxazine 4 has peak emission near into the NIR, which provided nerve-targeted signal in the brachial plexus and sciatic nerve for up to 12 h after a single intravenous injection. In addition, recurrent laryngeal nerves were successfully identified and highlighted in real time in swine, which could be preserved during the course of thyroid resection. Although optical properties of these agents are not yet optimal, chemical structure analysis provides a basis for improving these prototype nerve-specific NIR fluorophores even further.
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影响因子：
1.9
作者：
Shi, Chen;Flanagan, Steven R.;Samadani, Uzma
通讯作者：
Samadani, Uzma
DOI：
10.1016/j.jamcollsurg.2007.07.017
发表时间：
2008-01-01
影响因子：
5.2
作者：
Snyder, Samuel K.;Lairmore, Terry C.;Roberts, John W.
通讯作者：
Roberts, John W.
影响因子：
46.9
作者：
通讯作者：
--
影响因子：
2.8
作者：
Nakayama, Akira;Bianco, Antonio C;Frangioni, John V
通讯作者：
Frangioni, John V
影响因子：
2.8
作者：
Gibbs-Strauss, Summer L.;Nasr, Khaled A.;Frangioni, John V.
通讯作者：
Frangioni, John V.