Fluorescent peptides highlight peripheral nerves during surgery in mice.
Fluorescent peptides highlight peripheral nerves during surgery in mice.
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DOI：
10.1038/nbt.1764
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发表时间：
2011-04
影响因子：
46.9
通讯作者：
中科院分区：
文献类型：
--
作者：
神经保护是手术中的一个重要目标，因为意外的横断或损伤会导致严重的并发症，包括麻木、疼痛、虚弱或瘫痪。神经通常通过它们的外观和与附近结构的关系来识别，或者通过局部电刺激(肌电图术)来检测，但细小或埋藏的神经有时被忽视。在这里，我们使用噬菌体展示来选择优先与神经结合的多肽。在小鼠全身注射荧光标记的多肽后，2小时内所有周围神经均清晰可见，神经与周围组织的对比度可达10倍，有效对比度可达8小时。治疗后未观察到行为或活动的变化，表明没有明显的毒性。这种荧光探针还标记了人体组织样本中的神经。荧光突出显示与轴突的完整性无关，这表明该探头可以促进受损神经的手术修复，并有助于防止意外横断。
Nerve preservation is an important goal during surgery because accidental transection or injury leads to significant morbidity, including numbness, pain, weakness or paralysis. Nerves are usually identified by their appearance and relationship to nearby structures or detected by local electrical stimulation (electromyography), but thin or buried nerves are sometimes overlooked. Here, we use phage display to select a peptide that binds preferentially to nerves. After systemic injection of a fluorescently labeled version of the peptide in mice, all peripheral nerves are clearly delineated within 2 h. Contrast between nerve and adjacent tissue is up to tenfold, and useful contrast lasts up to 8 h. No changes in behavior or activity are observed after treatment, indicating a lack of obvious toxicity. The fluorescent probe also labels nerves in human tissue samples. Fluorescence highlighting is independent of axonal integrity, suggesting that the probe could facilitate surgical repair of injured nerves and help prevent accidental transection.
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影响因子：
64.8
作者：
Pasqualini, R;Ruoslahti, E
通讯作者：
Ruoslahti, E
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Berger, Sven;Bannantine, John P.;Griffin, J. Frank T.
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Griffin, J. Frank T.
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Gu, Jun
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Laszig, R
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RICHMOND, FJR;GLADDY, R;THOMSON, DB
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