Search for Microorganisms in Men with Urologic Chronic Pelvic Pain Syndrome: A Culture-Independent Analysis in the MAPP Research Network.
Search for Microorganisms in Men with Urologic Chronic Pelvic Pain Syndrome: A Culture-Independent Analysis in the MAPP Research Network.
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DOI：
10.1016/j.juro.2015.01.037
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发表时间：
2015-07
影响因子：
6.6
通讯作者：
Ehrlichk, Garth D.
中科院分区：
文献类型：
--
作者：
Nickel, J. Curtis;Stephens, Alisa;Landis, J. Richard;Chen, Jun;Mullins, Chris;van Bokhoven, Adrie;Lucia, M. Scott;Melton-Kreft, Rachael;Ehrlichk, Garth D.
我们使用新一代、最先进的、不依赖培养的方法来调查UCPPS男性和MAPP网络中登记的对照参与者的尿液微生物群，以调查可能的微生物病因。要求男性UCPPS患者和匹配的对照提供VB 1、VB 2和VB 3尿液标本。使用Ibis T-5000 Universal Biosensor技术分析样本，以提供细菌和选定真菌物种的全面鉴定。UCPPS和对照研究参与者之间的差异，存在的物种或物种的变异，在一个更高的分类分组（属）进行了评价，使用排列多变量方差分析和逻辑回归。从110名UCPPS参与者（67名VB 3）和115名对照者（62名VB 3）中获得VB 1和VB 2尿液标本。在VB 1、VB 2和VB 3中分别检测到78、73和54种（42、39和27属）。病例组和对照组的VB 1、VB 2和VB 3平均（SD）计数分别为1.62（1.28）、1.38（1.36）和1.33（1.24），分别为1.75（1.32）、1.23（1.15）和1.56（0.97）。在VB 1中，UCPPS和对照参与者之间的总体种属组成存在显著差异（p=0.002种属水平，p=0.004属水平），新洋葱伯克霍尔德菌在UCPPS病例中过度表达。在VB 2或VB 3样品中，在任何水平下均未观察到显著差异。对入组MAPP网络的男性受试者的基线培养非依赖性微生物学数据进行评估，发现UCPPS中B新洋葱头菌的代表性过高。未来的研究计划进一步评估微生物群与可变和不断变化的UCPPS症状模式的关系。
We used next-generation, state-of-the-art, culture-independent methodology to survey urine microbiota of UCPPS males and control participants enrolled in the MAPP Network to investigate a possible microbial etiology. Male UCPPS patients and matched controls were asked to provide VB1, VB2 and VB3 urine specimens. Specimens were analyzed with Ibis T-5000 Universal Biosensor technology to provide comprehensive identification of bacterial and select fungal species. Differences between UCPPS and control study participants for presence of species or species variation within a higher taxonomic grouping (genus) were evaluated using permutational multivariate analysis of variance and logistic regression. VB1 and VB2 urine specimens were obtained from 110 (VB3 in 67) UCPPS participants and 115 (VB3 in 62) controls. A total of 78, 73 and 54 species (42, 39 and 27 genera) were detected in VB1, VB2 and VB3 respectively. Mean (SD) VB1, VB2 and VB3 species count per person was 1.62 (1.28), 1.38 (1.36) and 1.33(1.24) for cases and 1.75(1.32), 1.23(1.15) and 1.56 (0.97) for controls respectively. Overall species and genus composition differed significantly between UCPPS and control participants in VB1 (p=0.002 species level, p=0.004 genus level) with Burkholderia cenocepacia over represented in UCPPS cases. No significant differences were observed at any level in VB2 or VB3 samples. Assessment of baseline culture-independent microbiological data from male subjects enrolled in the MAPP Network has identified over representation of B cenocepacia in UCPPS. Future studies are planned to further evaluate microbiota associations with variable and changing UCPPS symptom patterns.
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影响因子：
2.1
作者：
Keay, S;Zhang, CO;Alexander, RB
通讯作者：
Alexander, RB
影响因子：
3.1
作者：
Schwager, Stephan;Agnoli, Kirsty;Eberl, Leo
通讯作者：
Eberl, Leo
DOI：
10.1111/j.2517-6161.1995.tb02031.x
发表时间：
1995-01-01
影响因子：
5.8
作者：
BENJAMINI, Y;HOCHBERG, Y
通讯作者：
HOCHBERG, Y
影响因子：
5.3
作者：
Jacovides, Christina L.;Kreft, Rachael;Parvizi, Javad
通讯作者：
Parvizi, Javad
影响因子：
2.3
作者：
Palmer, Michael P.;Altman, Daniel T.;Costerton, John W.
通讯作者：
Costerton, John W.