The effect of osmolytes and small molecule on Quadruplex-WC duplex equilibrium: a fluorescence resonance energy transfer study.
The effect of osmolytes and small molecule on Quadruplex-WC duplex equilibrium: a fluorescence resonance energy transfer study.
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DOI：
10.1093/nar/gki961
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发表时间：
2005
影响因子：
14.9
通讯作者：
Maiti S
中科院分区：
文献类型：
--
作者：
Kumar N;Maiti S
G-四链体和沃森-克里克双链体之间的结构竞争与重复DNA序列有关，但在自然和药理学背景下影响这种竞争平衡的因素需要阐明。以21聚体的5′-黄绿素-d [（G3 TTA）3G 3]-塔姆拉-3′为模型体系，通过荧光共振能量转移分析，研究了该平衡对渗透胁迫和四链体选择性小分子的敏感性。在不同浓度的渗透剂（乙二醇和甘油）和四链体选择性配体（阳离子卟啉-TMPyP 4）的存在和不存在下，四链体与其互补链的杂交中所涉及的结合亲和力和动力学进行了测定。渗透剂和阳离子卟啉的存在下，降低了四链体的结合亲和力，其互补链，并通过延迟杂交过程减慢了反应的动力学。我们的结合数据分析表明，无论是渗透剂或卟啉的存在下，增加的量的四链体的平衡。在100 mM KCl溶液中，当将30 nM的各组分，即四链体和互补链混合在一起时，在不存在和存在10%乙二醇、10%甘油和150 nM TMPyP 4的情况下，平衡系统中存在的四链体的量分别为17.6、23.4、23.1和19.6 nM。在不存在和存在这些干扰剂的情况下的四链体的荧光熔解曲线证实了渗透剂和阳离子卟啉稳定四链体的发现，从而将平衡转移到四链体形成。
The structural competition between the G-quadruplex and Watson–Crick duplex has been implicated for the repetitive DNA sequences, but the factors influencing this competitive equilibrium in the natural and pharmacological context need to be elucidated. Using a 21mer 5′-Fluorescein-d[(G3TTA)3G3]-TAMRA-3′ as a model system, extensive fluorescence resonance energy transfer analysis was carried out to investigate sensitivity of this equilibrium to osmotic stress and quadruplex selective small molecule. The binding affinities and kinetics involved in the hybridization of quadruplex to its complementary strand in the absence and presence of different concentrations of osmolytes (ethylene glycol and glycerol) and a quadruplex selective ligand (cationic porphyrin-TMPyP4) were determined. The presence of osmolytes and cationic porphyrin decreased the binding affinity of quadruplex to its complementary strand and slowed the kinetics of the reaction by delaying the hybridization process. Our binding data analysis indicates that the presence of either osmolytes or porphyrin increase the amount of quadruplex in the equilibrium. In 100 mM KCl solution, when 30 nM of each of the components, i.e. quadruplex and the complementary strand, were mixed together, the amount of quadruplex present in the system under equilibrium were 17.6, 23.4, 23.1 and 19.6 nM in the absence and presence of 10% ethylene glycol, 10% glycerol and 150 nM TMPyP4, respectively. Fluorescence melting profile of quadruplex in the absence and presence of these perturbants confirm the findings that osmolytes and cationic porphyrin stabilize quadruplex, and thus, shift the equilibrium to quadruplex formation.
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影响因子：
4.8
作者：
Erlitzki, R;Fry, M
通讯作者：
Fry, M
影响因子：
2.9
作者：
Anantha, NV;Azam, M;Sheardy, RD
通讯作者：
Sheardy, RD
影响因子：
2.9
作者：
MARKY, LA;BRESLAUER, KJ
通讯作者：
BRESLAUER, KJ
DOI：
10.1016/j.bbrc.2004.05.052
发表时间：
2004-07-02
影响因子：
3.1
作者：
Kumar, N;Maiti, S
通讯作者：
Maiti, S
影响因子：
2.9
作者：
HARDIN, CC;WATSON, T;BAILEY, C
通讯作者：
BAILEY, C