Streptomyces venezuelae NRRL B-65442: genome sequence of a model strain used to study morphological differentiation in filamentous actinobacteria.
Streptomyces venezuelae NRRL B-65442: genome sequence of a model strain used to study morphological differentiation in filamentous actinobacteria.
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DOI：
10.1093/jimb/kuab035
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发表时间：
2021-12-23
影响因子：
3.4
通讯作者：
Bibb MJ
中科院分区：
文献类型：
--
作者：
Gomez-Escribano JP;Holmes NA;Schlimpert S;Bibb MJ;Chandra G;Wilkinson B;Buttner MJ;Bibb MJ
十多年来，委内瑞拉链霉菌已被用于研究控制链霉菌形态发育的分子机制，现在是一个完善的模型菌株。它的快速生长和在液体培养中以接近同步的方式形成孢子的能力，在链霉菌中是不寻常的，这极大地促进了现代分子技术的应用，如ChIP-seq和RNA-seq，以及完整链霉菌生命周期的延时荧光成像。在这里，我们描述了一个高质量的基因组序列，我们分离的菌株（北方区域研究实验室[NRRL] B-65442）的8.2 M B染色体和158 kb的质粒，pSVJI 1，这是以前没有报道过的。令人惊讶的是，NRRL B-65442在MYM琼脂上产生绿色孢子，而美国典型培养物保藏中心（ATCC）型菌株10712（NRRL B-65442来源于该菌株）产生灰色孢子。虽然两个分离物的基因组序列的比较显示几乎完全相同，但它确实揭示了参与孢子色素生物合成的基因vnz_33525中的单核苷酸取代。用NRRL B-65442的等位基因替换ATCC 10712的vnz_33525等位基因导致绿色孢子，解释了孢子色素沉着的差异。我们还应用CRISPR-Cas9来删除pSVJIl的必需parB，以从菌株中治愈质粒，而没有明显的表型后果。
For over a decade, Streptomyces venezuelae has been used to study the molecular mechanisms that control morphological development in streptomycetes and is now a well-established model strain. Its rapid growth and ability to sporulate in a near-synchronised manner in liquid culture, unusual among streptomycetes, greatly facilitates the application of modern molecular techniques such as ChIP-seq and RNA-seq, as well as time-lapse fluorescence imaging of the complete Streptomyces life cycle. Here we describe a high-quality genome sequence of our isolate of the strain (Northern Regional Research Laboratory [NRRL] B-65442) consisting of an 8.2 Mb chromosome and a 158 kb plasmid, pSVJI1, which had not been reported previously. Surprisingly, while NRRL B-65442 yields green spores on MYM agar, the American Type Culture Collection (ATCC) type strain 10712 (from which NRRL B-65442 was derived) produces grey spores. While comparison of the genome sequences of the two isolates revealed almost total identity, it did reveal a single nucleotide substitution in a gene, vnz_33525, involved in spore pigment biosynthesis. Replacement of the vnz_33525 allele of ATCC 10712 with that of NRRL B-65442 resulted in green spores, explaining the discrepancy in spore pigmentation. We also applied CRISPR-Cas9 to delete the essential parB of pSVJI1 to cure the plasmid from the strain without obvious phenotypic consequences.
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影响因子：
6.4
作者：
Bush MJ;Bibb MJ;Chandra G;Findlay KC;Buttner MJ
通讯作者：
Buttner MJ
DOI：
10.1007/s10295-006-0113-4
发表时间：
2006-09-01
影响因子：
3.4
作者：
Jakeman, David L.;Graham, Cathy L.;Vining, Leo C.
通讯作者：
Vining, Leo C.
影响因子：
3.6
作者：
Haist, Julian;Neumann, Sara Alina;Tschowri, Natalia
通讯作者：
Tschowri, Natalia
影响因子：
2.6
作者：
Hall, Steven R;Blundon, Heather L;Ladda, Matthew A;Robertson, Andrew W;Martinez-Farina, Camilo F;Jakeman, David L;Goralski, Kerry B
通讯作者：
Goralski, Kerry B
影响因子：
5
作者：
Funnell BE
通讯作者：
Funnell BE