Comparative genome analysis of Lactobacillus rhamnosus clinical isolates from initial stages of dental pulp infection: identification of a new exopolysaccharide cluster.
Comparative genome analysis of Lactobacillus rhamnosus clinical isolates from initial stages of dental pulp infection: identification of a new exopolysaccharide cluster.
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DOI：
10.1371/journal.pone.0090643
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Hunter N
中科院分区：
文献类型：
--
作者：
Nadkarni MA;Chen Z;Wilkins MR;Hunter N
人类口腔微生物组在包括龋齿在内的口腔疾病中起着重要作用。我们的研究进展龋齿感染通过牙本质和最近，侵入活髓，检测鼠李糖乳杆菌感染的初始阶段的活髓组织。在这项研究中，采用目前的高通量下一代测序技术，我们试图获得深入了解组织入侵L。鼠李糖乳杆菌，以识别生物标志物，可以提供对乳杆菌致病潜力的理解，通常被认为是安全的。采用Roche GS FLX+技术对两株临床分离的乳杆菌进行全基因组测序。鼠李糖杆菌感染活髓。详细的全基因组比较的遗传图谱的组织入侵L。益生菌L. rhamnosus进行测试的假设，特定菌株的L。选择具有独特基因互补的鼠李糖乳杆菌以获得侵入活髓组织的能力。分析分别鉴定了264个和258个基因，从牙髓侵袭性L。从两个不同受试者分离的鼠李糖菌株LRHMDP 2和LRHMDP 3，其不存在于参考益生菌L中。鼠李糖菌株ATCC 53103（GG）。鉴定的不同基因组特征包括修饰的胞外多糖簇的存在，这一特征在另外6个临床分离株中得到证实。LRHMDP 2和LRHMDP 3的其他特征是来自RpoN、NtrC、MutR、ArsR和锌结合Cro/CI家族的转录调节因子的改变，以及双组分传感器激酶反应调节因子和三价铁的ABC转运蛋白的变化。两株临床分离的L. rhamnosus与L. rhamnosusLc 705，而不是L.鼠李糖酵母ATCC 53103（GG）。基因组距离分析和SNP分歧证实了临床分离株的密切关系，但与参考益生菌L分离。鼠李糖菌株ATCC 53103（GG）。
The human oral microbiome has a major role in oral diseases including dental caries. Our studies on progression of caries infection through dentin and more recently, the invasion of vital dental pulp, detected Lactobacillus rhamnosus in the initial stages of infection of vital pulp tissue. In this study employing current high-throughput next generation sequencing technology we sought to obtain insight into genomic traits of tissue invasive L. rhamnosus, to recognise biomarkers that could provide an understanding of pathogenic potential of lactobacilli, generally regarded as safe. Roche GS FLX+ technology was used to generate whole genome sequences of two clinical isolates of L. rhamnosus infecting vital pulp. Detailed genome-wide comparison of the genetic profiles of tissue invasive L. rhamnosus with probiotic L. rhamnosus was performed to test the hypothesis that specific strains of L. rhamnosus possessing a unique gene complement are selected for the capacity to invade vital pulp tissue. Analysis identified 264 and 258 genes respectively, from dental pulp-invasive L. rhamnosus strains LRHMDP2 and LRHMDP3 isolated from two different subjects that were not present in the reference probiotic L. rhamnosus strain ATCC 53103 (GG). Distinct genome signatures identified included the presence of a modified exopolysaccharide cluster, a characteristic confirmed in a further six clinical isolates. Additional features of LRHMDP2 and LRHMDP3 were altered transcriptional regulators from RpoN, NtrC, MutR, ArsR and zinc-binding Cro/CI families, as well as changes in the two-component sensor kinase response regulator and ABC transporters for ferric iron. Both clinical isolates of L. rhamnosus contained a single SpaFED cluster, as in L. rhamnosus Lc705, instead of the two Spa clusters (SpaCBA and SpaFED) identified in L. rhamnosus ATCC 53103 (GG). Genomic distance analysis and SNP divergence confirmed a close relationship of the clinical isolates but segregation from the reference probiotic L. rhamnosus strain ATCC 53103 (GG).
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影响因子：
3.3
作者：
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通讯作者：
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DOI：
10.1073/pnas.0908876106
发表时间：
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影响因子：
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DOI：
10.1073/pnas.1000086107
发表时间：
2011-03-15
影响因子：
11.1
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