Endophytic fungal association via gibberellins and indole acetic acid can improve plant growth under abiotic stress: an example of Paecilomyces formosus LHL10.
Endophytic fungal association via gibberellins and indole acetic acid can improve plant growth under abiotic stress: an example of Paecilomyces formosus LHL10.
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DOI：
10.1186/1471-2180-12-3
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发表时间：
2012-01-12
期刊：
影响因子：
4.2
通讯作者：
Lee IJ
中科院分区：
文献类型：
--
作者：
Khan AL;Hamayun M;Kang SM;Kim YH;Jung HY;Lee JH;Lee IJ
内生真菌在外源分泌植物激素和缓解盐胁迫方面所知甚少，这是世界范围内农业生产的主要限制因素。本研究旨在从黄瓜根系中分离出产生植物激素的内生真菌，并确定其在盐碱条件下植物生长和耐胁迫中的作用。从黄瓜根中分离到9种内生真菌，并在赤霉素（ghberellins, GAs）缺乏的突变型水稻Waito-C和正常的GAs生物合成型水稻Dongjin-byeo上筛选了它们的培养滤液。与对照相比，真菌分离物CSH-6H的CF显著促进了Waito-C和Dongjin-byeo幼苗的生长。CF分析显示存在GAs （GA1、GA3、GA4、GA8、GA9、GA12、GA20和GA24）和吲哚乙酸。基于ITS序列相似性的系统发育分析，将内生菌CSH-6H鉴定为台湾拟青霉LHL10菌株。在盐胁迫下，与未接种的对照植株相比，接种台湾青松显著提高了黄瓜的茎长和相关生长特性。在寄主植物根的皮质区和中柱周区也观察到福尔莫斯的菌丝，并利用PCR技术成功地重新分离了菌丝。台塑结合力通过积累脯氨酸和抗氧化剂，维持植物水势来抵消盐度的不利影响。因此，在内生菌的作用下，黄瓜植株的电解泄漏和膜损伤减少。胁迫响应性脱落酸含量的降低表明内生植物受到的胁迫较少。相反，黄瓜内生菌相关植株的内源气体（GA3、GA4、GA12和GA20）含量升高，表明盐胁迫调节了植株的生长。结果表明，内生真菌分泌的植物激素相互作用可以改善寄主植物的生长，减轻盐胁迫的不利影响。该真菌菌株可用于进一步的田间试验，以提高盐碱条件下的农业生产力。
Endophytic fungi are little known for exogenous secretion of phytohormones and mitigation of salinity stress, which is a major limiting factor for agriculture production worldwide. Current study was designed to isolate phytohormone producing endophytic fungus from the roots of cucumber plant and identify its role in plant growth and stress tolerance under saline conditions. We isolated nine endophytic fungi from the roots of cucumber plant and screened their culture filtrates (CF) on gibberellins (GAs) deficient mutant rice cultivar Waito-C and normal GAs biosynthesis rice cultivar Dongjin-byeo. The CF of a fungal isolate CSH-6H significantly increased the growth of Waito-C and Dongjin-byeo seedlings as compared to control. Analysis of the CF showed presence of GAs (GA1, GA3, GA4, GA8, GA9, GA12, GA20 and GA24) and indole acetic acid. The endophyte CSH-6H was identified as a strain of Paecilomyces formosus LHL10 on the basis of phylogenetic analysis of ITS sequence similarity. Under salinity stress, P. formosus inoculation significantly enhanced cucumber shoot length and allied growth characteristics as compared to non-inoculated control plants. The hypha of P. formosus was also observed in the cortical and pericycle regions of the host-plant roots and was successfully re-isolated using PCR techniques. P. formosus association counteracted the adverse effects of salinity by accumulating proline and antioxidants and maintaining plant water potential. Thus the electrolytic leakage and membrane damage to the cucumber plants was reduced in the association of endophyte. Reduced content of stress responsive abscisic acid suggest lesser stress convened to endophyte-associated plants. On contrary, elevated endogenous GAs (GA3, GA4, GA12 and GA20) contents in endophyte-associated cucumber plants evidenced salinity stress modulation. The results reveal that mutualistic interactions of phytohormones secreting endophytic fungi can ameliorate host plant growth and alleviate adverse effects of salt stress. Such fungal strain could be used for further field trials to improve agricultural productivity under saline conditions.
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影响因子：
6.1
作者：
Hamayun, Muhammad;Khan, Sumera Afzal;Lee, In-Jung
通讯作者：
Lee, In-Jung
影响因子：
2.8
作者：
Arnold, A. Elizabeth;Henk, Daniel A.;Vilgalys, Rvtas
通讯作者：
Vilgalys, Rvtas
影响因子：
3.9
作者：
Augé, RM;Moore, JL;Cho, KH
通讯作者：
Cho, KH
DOI：
10.1007/978-3-642-01979-1_1
发表时间：
2009-01-01
期刊：
MICROBIAL STRATEGIES FOR CROP IMPROVEMENT
影响因子：
--
作者：
Gamalero, Elisa;Berta, Graziella;Glick, Bernard R.
通讯作者：
Glick, Bernard R.
影响因子：
6.9
作者：
Jiang, MY;Zhang, JH
通讯作者：
Zhang, JH