Hyperpolarization‐activated (Ih) currents in auditory brainstem neurons of normal and congenitally deaf mice
Hyperpolarization‐activated (Ih) currents in auditory brainstem neurons of normal and congenitally deaf mice
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正常和先天性耳聋小鼠听觉脑干神经元的超极化激活（Ih）电流
DOI：
10.1111/j.1460-9568.2005.04185.x
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发表时间：
2005
影响因子：
3.4
通讯作者：
B. Walmsley
中科院分区：
文献类型：
--
作者：
R. Leão;Katarina Svahn;A. Berntson;B. Walmsley
我们研究了先天性耳聋 (dn/dn) 小鼠模型中脑干听觉神经元的膜特性。全细胞记录是由梯形体内侧核（MNTB）和耳蜗前腹核（AVCN）切片中的可视化神经元进行的。我们最近证明，耳聋小鼠的 MNTB 神经元比正常小鼠更兴奋，部分原因是低阈值钾电流的表达减少。在这项研究中，我们研究了超极化激活 (Ih) 通道对 MNTB 和 AVCN 神经元膜特性的贡献。我们的结果表明，耳聋小鼠的 MNTB 神经元中的 Ih 比正常小鼠的更大。相反，来自 dn/dn 和正常小鼠的 AVCN 浓密细胞的 Ih 或兴奋性没有显着差异。实验证据和神经元模型表明，在正常小鼠的 MNTB 中，Ih 的一小部分贡献有助于减少突触电位的时间总和。聋鼠神经元中较大的 Ih 对减少突触电位的时间总和具有更大的影响，在一定程度上抵消了这些细胞的较高兴奋性。我们的结果进一步深入了解了发育过程中活动在调节中枢神经元膜和放电特性中的作用。
We have investigated the membrane properties of brainstem auditory neurons in a mouse model of congenital deafness (dn/dn). Whole‐cell recordings were made from visualized neurons in slices of the medial nucleus of the trapezoid body (MNTB) and anteroventral cochlear nucleus (AVCN). We have recently demonstrated that MNTB neurons in deaf mice are more excitable than in normal mice, due in part to a reduced expression of low‐threshold potassium currents. In this study, we have examined the contribution of hyperpolarization‐activated (Ih) channels to the membrane properties of MNTB and AVCN neurons. Our results show that Ih is larger in MNTB neurons from deaf mice than in normal mice. In contrast, no significant differences were found in Ih or excitability between AVCN bushy cells from dn/dn and normal mice. Experimental evidence and neuronal modelling suggests that, in the MNTB of normal mice, a small contribution of Ih helps to reduce temporal summation of synaptic potentials. A larger Ih in neurons from deaf mice has a much greater effect in reducing temporal summation of synaptic potentials, counteracting to some extent the greater excitability of these cells. Our results provide further insight into the role of activity during development in regulating the membrane and firing properties of central neurons.
影响因子：
2.5
作者：
BANKS, MI;PEARCE, RA;SMITH, PH
通讯作者：
SMITH, PH
影响因子：
56.9
作者：
CASSEDAY, JH;EHRLICH, D;COVEY, E
通讯作者：
COVEY, E
影响因子：
2.5
作者：
Bal, R;Oertel, D
通讯作者：
Oertel, D