Mathematical modeling of diurnal patterns of carbon allocation to shoot and root in Arabidopsis thaliana
Mathematical modeling of diurnal patterns of carbon allocation to shoot and root in Arabidopsis thaliana
复制标题
拟南芥芽和根碳分配昼夜模式的数学模型
DOI：
10.1038/s41540-018-0080-1
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发表时间：
2019
影响因子：
4
通讯作者：
Nägele
中科院分区：
文献类型：
--
作者：
Küstner;Nägele
我们开发了一个数学模型来模拟暴露于正常（120 µmol m−2s−1）或高光照强度（1200 µ mol m − 2s−1）的拟南芥叶片在完整的昼夜周期中中心碳代谢的动态。主要目的是获得一个高分辨率的时间序列代谢动力学以及拍摄结构碳的形成（化合物具有较长的停留时间）和同化出口的气生器官的汇组织。模型开发包括逐步增加复杂性，最终接近体内情况。同化物的正确分配，无论是汇出口或拍摄结构碳的形成是一个中心目标的模型开发。结构碳的日增益是基于总光合固碳的日增量计算的，这是模型中实现的结构碳形成的唯一参数。中央代谢物池的动态模拟显示，拍摄结构碳的形成只发生在光相，但不是在夜间。该模型允许模拟拍摄结构碳形成的功能，在不同的环境条件下，没有结构修改的中央叶碳代谢。对登录Landsbergerecta（Ler）及其己糖激酶null-muplogin 2 -1进行模型模拟。该突变体表现出生长缓慢的表型，特别是在增加光照强度时。模拟结果的比较表明，延迟的突变体的地上部生长增加同化物运输到库器官。由于其在蔗糖循环和糖信号传导中的中心功能，我们的研究结果表明，己糖激酶-1对于碳分配到芽生长或同化物输出中的重要作用。
We developed a mathematical model to simulate dynamics of central carbon metabolism over complete diurnal cycles for leaves ofArabidopsis thalianaexposed to either normal (120 µmol m−2s−1) or high light intensities (1200 µmol m−2s−1). The main objective was to obtain a high-resolution time series for metabolite dynamics as well as for shoot structural carbon formation (compounds with long residence time) and assimilate export of aerial organs to the sink tissue. Model development comprised a stepwise increment of complexity to finally approach thein vivosituation. The correct allocation of assimilates to either sink export or shoot structural carbon formation was a central goal of model development. Diurnal gain of structural carbon was calculated based on the daily increment in total photosynthetic carbon fixation, and this was the only parameter for structural carbon formation implemented in the model. Simulations of the dynamics of central metabolite pools revealed that shoot structural carbon formation occurred solely during the light phase but not during the night. The model allowed simulation of shoot structural carbon formation as a function of central leaf carbon metabolism under different environmental conditions without structural modifications. Model simulations were performed for the accession Landsbergerecta(Ler) and its hexokinase null-mutantgin2-1. This mutant displays a slow growth phenotype especially at increasing light intensities. Comparison of simulations revealed that the retarded shoot growth in the mutant resulted from an increased assimilate transport to sink organs. Due to its central function in sucrose cycling and sugar signaling, our findings suggest an important role of hexokinase-1 for carbon allocation to either shoot growth or assimilate export.
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影响因子：
5.1
作者：
Ruts T;Matsubara S;Walter A
通讯作者：
Walter A
影响因子：
9.5
作者：
Li L;Sheen J
通讯作者：
Sheen J
影响因子：
7.2
作者：
Gibon, Y;Vigeolas, H;Stitt, M
通讯作者：
Stitt, M
菠菜叶蔗糖磷酸合成酶的纯化和性质：该酶和果糖双磷酸酶参与蔗糖生物合成的调节。
影响因子：
3.9
作者：
S. Harbron;C. Foyer;D. Walker
通讯作者：
D. Walker
Clusia spp 的昼夜生长周期。
DOI：
--
发表时间：
2008
期刊：
Plant, Cell and Environment
影响因子：
--
作者：
A. Walter;M. M. Christ;U. Rascher;U. Schurr;B. Osmond
通讯作者：
B. Osmond