Synchronous high-resolution phenotyping of leaf and root growth in Nicotiana tabacum over 24-h periods with GROWMAP-plant.
Synchronous high-resolution phenotyping of leaf and root growth in Nicotiana tabacum over 24-h periods with GROWMAP-plant.
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DOI：
10.1186/1746-4811-9-2
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发表时间：
2013-01-23
期刊：
影响因子：
5.1
通讯作者：
Walter A
中科院分区：
文献类型：
--
作者：
Ruts T;Matsubara S;Walter A
根系生长对环境的时间变化有高度的反应。相反，双叶植物叶片扩张的diel （24 h）受内源控制，因此其时间模式并不严格遵循环境中的diel变化。然而，根和茎通过资源分配相互联系，一个器官的环境变化会影响另一个器官的生长，从而影响植物的整体生长。我们开发了一种新的技术，GROWMAP-plant，以高分辨率监测同一植物叶片和根系的生长过程。这使我们能够量化治疗对治疗和未治疗器官生长速度的影响，以及它们之间可能的相互作用。我们将烟草幼苗根系置于三种不同的条件下：22°C持续黑暗（对照）、10°C持续黑暗（根冷却）和22°C 12 h/12 h光暗循环（根照明）。在所有处理中，在22°C下保持相同的12 h/12 h明暗循环。根区环境保持不变时，根系生长速率不变，但根系冷却处理显著降低了根系生长。根系光照处理后，根速降低，导致根生长有节律性。尽管根生长发生了这些变化，但根区处理对叶片生长没有实质性影响，其夜间生长持续表现为昼夜生长的三倍。GROWMAP-plant允许在同一株植物中对叶和根进行详细的同步生长表型分析。根系冷却和光照对幼苗叶片的相对生长速率和生长模式均无显著影响。我们在同一植物的生长叶片和根中同时获得的结果证实了根生长对环境的高度敏感性以及双科植物叶片中diel生长模式的对比稳健性。此外，它们还强调了仔细控制根生长分析的实验条件以避免或/和减少人工并发症的重要性。
Root growth is highly responsive to temporal changes in the environment. On the contrary, diel (24 h) leaf expansion in dicot plants is governed by endogenous control and therefore its temporal pattern does not strictly follow diel changes in the environment. Nevertheless, root and shoot are connected with each other through resource partitioning and changing environments for one organ could affect growth of the other organ, and hence overall plant growth. We developed a new technique, GROWMAP-plant, to monitor growth processes synchronously in leaf and root of the same plant with a high resolution over the diel period. This allowed us to quantify treatment effects on the growth rates of the treated and non-treated organ and the possible interaction between them. We subjected the root system of Nicotiana tabacum seedlings to three different conditions: constant darkness at 22°C (control), constant darkness at 10°C (root cooling), and 12 h/12 h light–dark cycles at 22°C (root illumination). In all treatments the shoot was kept under the same 12 h/12 h light–dark cycles at 22°C. Root growth rates were found to be constant when the root-zone environment was kept constant, although the root cooling treatment significantly reduced root growth. Root velocity was decreased after light-on and light-off events of the root illumination treatment, resulting in diel root growth rhythmicity. Despite these changes in root growth, leaf growth was not affected substantially by the root-zone treatments, persistently showing up to three times higher nocturnal growth than diurnal growth. GROWMAP-plant allows detailed synchronous growth phenotyping of leaf and root in the same plant. Root growth was very responsive to the root cooling and root illumination, while these treatments altered neither relative growth rate nor diel growth pattern in the seedling leaf. Our results that were obtained simultaneously in growing leaves and roots of the same plants corroborate the high sensitivity of root growth to the environment and the contrasting robustness of diel growth patterns in dicot leaves. Further, they also underpin the importance to carefully control the experimental conditions for root growth analysis to avoid or/and minimize artificial complications.
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影响因子：
6.9
作者：
ABBASALANI, MK;HAY, RKM
通讯作者：
HAY, RKM
影响因子：
4.2
作者：
Iijima, M;Oribe, Y;Kono, Y
通讯作者：
Kono, Y
影响因子：
4.3
作者：
Iijima, Morio;Matsushita, Naofumi
通讯作者：
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影响因子：
11.6
作者：
Pyl, Eva-Theresa;Piques, Maria;Sulpice, Ronan
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影响因子：
6.4
作者：
ELIASSON, L;BOLLMARK, M
通讯作者：
BOLLMARK, M