Rapid detection of human immunodeficiency virus type 1 group M by a reverse transcription-loop-mediated isothermal amplification assay.
Rapid detection of human immunodeficiency virus type 1 group M by a reverse transcription-loop-mediated isothermal amplification assay.
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DOI：
10.1016/j.jviromet.2009.01.004
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发表时间：
2009-05
影响因子：
3.1
通讯作者：
Ichimura H
中科院分区：
文献类型：
--
作者：
Hosaka N;Ndembi N;Ishizaki A;Kageyama S;Numazaki K;Ichimura H
建立了一种针对pol-integrase基因的逆转录-环介导等温扩增（RT-LAMP）一步法快速检测HIV-1 M组的方法。这种HIV-1 RT-LAMP检测方法简单快速，在60 ℃等温条件下，35分钟内即可完成扩增。使用标准毒株（WHO HIV-1 [97/656]）在八周内测定HIV-1 RT-LAMP的100%检测限，结果为120拷贝/ml。使用从喀麦隆的57名HIV-1感染者和40名未感染者中采集的血浆样本评价了RT-LAMP检测法用于临床诊断的用途，喀麦隆的HIV-1毒株流行差异很大。在57份来自感染者的样本中，56份携带M组HIV-1毒株，如亚型A、B、G、F2和循环重组型（CRF）_01、_02、_09、_11、_13;所有样本均为RT-LAMP阳性。1份携带O型HIV-1的样本和40份未感染HIV-1的样本均为RT-LAMP阴性。这些结果表明，HIV-1 RT-LAMP可以快速检测HIV-1 M组RNA，并具有较高的敏感性和特异性。这些数据还表明，这种RT-LAMP检测可用于确认HIV诊断，特别是在资源有限的环境中。
A rapid one-step reverse transcription-loop-mediated isothermal amplification (RT-LAMP) assay targeting the pol-integrase gene was developed to detect human immunodeficiency virus type 1 (HIV-1) group M. This HIV-1 RT-LAMP assay is simple and rapid, and amplification can be completed within 35 min under isothermal conditions at 60 °C. The 100% detection limit of HIV-1 RT-LAMP was determined using a standard strain (WHO HIV-1 [97/656]) in octuplicate and found to be 120 copies/ml. The RT-LAMP assay was evaluated for use for clinical diagnosis using plasma samples collected from 57 HIV-1-infected and 40 uninfected individuals in Cameroon, where highly divergent HIV-1 strains are prevalent. Of the 57 samples from infected individuals, 56 harbored group-M HIV-1 strains, such as subtypes A, B, G, F2, and circulating recombinant forms (CRFs) _01, _02, _09, _11, _13; all were RT-LAMP positive. One sample harboring group-O HIV-1 and the 40 HIV-1-uninfected samples were RT-LAMP negative. These findings indicate that HIV-1 RT-LAMP can detect HIV-1 group-M RNA from plasma samples rapidly and with high sensitivity and specificity. These data also suggest that this RT-LAMP assay can be useful for confirming HIV diagnosis, particularly in resource-limited settings.
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影响因子：
9.4
作者：
Parida, M;Posadas, G;Morita, K
通讯作者：
Morita, K
影响因子：
3.1
作者：
Davis, C;Heath, A;Holmes, H
通讯作者：
Holmes, H
影响因子：
12.7
作者：
Ushio, M;Yui, I;Nakayama, T
通讯作者：
Nakayama, T
DOI：
10.1097/00126334-200412150-00019
发表时间：
2004-12-15
影响因子：
3.6
作者：
Ndembi, N;Takehisa, J;Ichimura, H
通讯作者：
Ichimura, H
影响因子：
9.4
作者：
Okafuji, T;Yoshida, N;Nakayama, T
通讯作者：
Nakayama, T