Pressing Intervention Promotes the Skeletal Muscle Repair of Traumatic Myofascial Trigger Points in Rats.
Pressing Intervention Promotes the Skeletal Muscle Repair of Traumatic Myofascial Trigger Points in Rats.
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DOI：
10.2147/jpr.s333705
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发表时间：
2021
影响因子：
2.7
通讯作者：
Li J
中科院分区：
文献类型：
--
作者：
Jiang Q;Feng X;Liu D;Li T;Kuang X;Liu X;Li W;Li J
观察按压干预对大鼠肌筋膜触发点骨骼肌修复的影响，探讨按压干预触发点失活的机制。将30只SPF级大鼠随机分为空白组、模型组和应激组，每组10只。采用钝性打击加离心运动的方法建立大鼠MTrPs模型，并进行评价。挤压组采用自制的挤压干预装置进行挤压干预14 d，其余两组正常喂养。分别于干预前后测量软组织张力（STT）D0.2和压痛阈值（PPT）。提取MTrPs时的骨骼肌组织，并通过苏木精-伊红（HE）和Masson染色进行评估。Western Blotting检测I型胶原、III型胶原、α-平滑肌肌动蛋白（α-SMA）、肌球蛋白重链（MHC）和纤维连接蛋白（FN）的表达。酶联免疫吸附试验（ELISA）检测P物质（SP）、5-羟色胺（5-HT）、环氧合酶2（考克斯-2）和前列腺素E2（PGE 2）的表达。(1)与空白组比较，模型组PPT、D0.2降低（P < 0.05），而挤压组PPT、D0.2升高（P < 0.05）。(2)与空白组比较，模型组出现明显的自发电位，其幅值和频率均明显增高，也明显高于按压组（P < 0.05）。(3)HE和Masson染色结果显示，模型组肌组织纤维化明显，胶原纤维面积较压迫组大（P < 0.05）。(4)与空白组比较，模型组I型胶原、III型胶原、FN、α- SMA、SP、5-HT、考克斯-2和PGE 2含量增加，MHC含量降低（P <0.05），而挤压组各项物质含量均较模型组降低（P <0.05），但MHC含量未增加（P < 0.05）。压迫干预大鼠肌筋膜后膜可以减轻慢性炎症，抑制纤维化，促进骨骼肌修复，减轻疼痛。
To observe the effect of pressing intervention on the skeletal muscle repair of myofascial trigger points (MTrPs) in rats and explore the mechanism of pressing intervention on the deactivation of trigger points. Thirty SPF rats were randomly divided into blank group, model group and press group, with 10 rats in each group. The MTrPs models were established by blunt striking plus eccentric exercise, and then evaluated. The press group was given a pressing intervention with a self-made device for 14 days, and the rats in the other two groups were fed normally. Soft tissue tension (STT) D0.2 and pressure pain threshold (PPT) were measured before and after intervention. The skeletal muscle tissue at MTrPs was extracted and assessed by hematoxylin–eosin (HE) and Masson staining. The expression of collagen I, collagen III, α- smooth muscle actin (α-SMA), myosin heavy chain (MHC) and fibronectin (FN) were detected by Western Blotting. Enzyme linked immunosorbent assay (ELISA) was used to evaluate the expression of substance P (SP), 5-hydroxytryptamine (5-HT), cyclooxygenase 2 (COX-2) and prostaglandin E2 (PGE2). (1) Compared with the blank group, the PPT and D0.2 reduced (P < 0.05) in the model group; while compared with the model group, the PPT and D0.2 increased (P < 0.05) in the press group. (2) Compared with the blank group, the model group showed obvious spontaneous potentials with higher amplitude and frequency, which were also much higher than those of the press group (P < 0.05). (3) The HE and Masson staining results showed evident fibrosis in the muscle tissue of the model group, with a larger area of collagen fibers relative to that of the press group (P < 0.05). (4) The amount of collagen I, collagen III, FN, α- SMA, SP, 5-HT, COX-2 and PGE2 increased and the content of MHC decreased (P < 0.05) in the model group, as compared to the blank group; while all the substances (P < 0.05), instead of MHC which increased (P < 0.05), in the press group were decreased as compared to the model group. Pressing intervention on the MTrPs in rats can alleviate chronic inflammation, inhibit fibrosis, promote skeletal muscle repair and relieve pain.
DOI：
10.1155/2013/869398
发表时间：
2013
期刊：
Evidence-based complementary and alternative medicine : eCAM
影响因子：
--
作者：
Wang R;Luo D;Xiao C;Lin P;Liu S;Xu Q;Wang Y
通讯作者：
Wang Y