The time course effects of electroacupuncture on promoting skeletal muscle regeneration and inhibiting excessive fibrosis after contusion in rabbits.
The time course effects of electroacupuncture on promoting skeletal muscle regeneration and inhibiting excessive fibrosis after contusion in rabbits.
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DOI：
10.1155/2013/869398
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Wang Y
中科院分区：
文献类型：
--
作者：
Wang R;Luo D;Xiao C;Lin P;Liu S;Xu Q;Wang Y
本研究旨在观察电针足三里、阿是穴对兔挫伤后骨骼肌再生和过度纤维化的纵向影响。将60只家兔随机分为正常组、挫伤组、电针组和重组人胰岛素样生长因子-I（rhIGF-I）组。急性骨骼肌挫伤产生的右腓肠肌（GM）的仪器为基础的滴质量技术。每两天用0.4 mA（2 Hz）进行EA 15 min，并用rhIGF-I（0.25 mL，每周一次）进行GM注射。电针组家兔血清T-SOD、T-AOC活性升高，MDA水平降低，血液灌流量明显增加。电针组肌原纤维直径均匀，排列规则，挫伤组则相反。电针组再生肌纤维数目、直径增加，MHC表达增加。电针治疗显著减少损伤肌肉的纤维化形成，并降低GDF-8和p-Smad 2/3的表达。我们的数据表明，EA可以通过改善血流和抗氧化能力来促进肌纤维再生并减少过度纤维化。此外，电针还可调节脑挫伤后信号传导因子的表达。
The aim of this study was to investigate the longitudinal effects of electroacupuncture (EA) on Zusanli (ST36) and Ashi acupoints in promoting skeletal muscle regeneration and inhibiting excessive fibrosis after contusion in rabbits. Sixty rabbits were randomly divided into four groups: normal, contusion, EA, and recombinant human insulin-like growth factor-I (rhIGF-I). An acute skeletal muscle contusion was produced on the right gastrocnemius (GM) by an instrument-based drop-mass technique. EA was performed for 15 minutes every two days with 0.4 mA (2 Hz), and GM injections were executed with rhIGF-I (0.25 mL once a week). Rabbits treated with EA had a higher T-SOD and T-AOC serum activities and lower MDA serum level, the blood perfusion of which was also significantly higher. In the EA group, the diameter of the myofibril was uniform and the arrangement was regular, contrary to the contusion group. The number and diameter of regenerative myofibers and MHC expression were increased in the EA group. EA treatment significantly decreased fibrosis formation and reduced both GDF-8 and p-Smad2/3 expressions in injured muscle. Our data indicate that EA may promote myofiber regeneration and reduce excessive fibrosis by improving blood flow and antioxidant capacities. Additionally, EA may regulate signaling factor expression after contusion.
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DOI：
10.1152/ajpendo.00495.2010
发表时间：
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影响因子：
5.1
作者：
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