Genome-wide identification and expression analysis of dirigent-jacalin genes from plant chimeric lectins in Moso bamboo (Phyllostachys edulis).
Genome-wide identification and expression analysis of dirigent-jacalin genes from plant chimeric lectins in Moso bamboo (Phyllostachys edulis).
复制标题
毛竹（Phyllostachys edulis）植物嵌合凝集素的 dirigent-jacalin 基因的全基因组鉴定和表达分析。
DOI：
10.1371/journal.pone.0248318
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发表时间：
2021
期刊：
影响因子：
3.7
通讯作者：
Zhang Z
中科院分区：
文献类型：
--
作者：
Ma R;Huang B;Chen J;Huang Z;Yu P;Ruan S;Zhang Z
Dirigent-Jacalin(D-J)基因属于植物嵌合凝集素家族，在植物生长、抗非生物逆境和生物逆境中发挥重要作用。为了探讨D-J家族在毛竹生长发育中的作用，对D-J家族的理化性质、系统发育关系、基因和蛋白质结构以及表达模式进行了详细分析。在毛竹基因组中发现了4个可能的PED-J基因，微序列分析和系统发育分析表明它们代表了植物凝集素进化中的一个新分支。PED-J蛋白由一个引导域和一个与JAX相关的凝集素结构域组成，每个结构域都包含两个不同的基序。多序列比对和同源模建分析表明，PED-J蛋白的三维结构与其他植物凝集素显著不同，这主要是由于其串联的引导域和JAXALIN结构域。我们调查了PED-Js上游可能的启动子区域，发现它们主要包含与激素和非生物应激反应相关的顺式作用元件。根、叶、根茎和穗部的表达谱分析表明，4个PED-J基因在穗部高表达，表明它们可能在毛竹几种不同组织类型的形成和发育过程中都是必需的。此外，PED-J基因与竹笋的快速生长发育有关。实时定量聚合酶链式反应(qRT-PCR)进一步验证了D-J家族基因对激素和胁迫的响应。本研究结果将有助于阐明PED-Js在竹子生长发育和逆境响应中的生物学功能。
Dirigent-jacalin (D-J) genes belong to the plant chimeric lectin family, and play vital roles in plant growth and resistance to abiotic and biotic stresses. To explore the functions of the D-J family in the growth and development of Moso bamboo (Phyllostachys edulis), their physicochemical properties, phylogenetic relationships, gene and protein structures, and expression patterns were analyzed in detail. Four putative PeD-J genes were identified in the Moso bamboo genome, and microsynteny and phylogenetic analyses indicated that they represent a new branch in the evolution of plant lectins. PeD-J proteins were found to be composed of a dirigent domain and a jacalin-related lectin domain, each of which contained two different motifs. Multiple sequence alignment and homologous modeling analysis indicated that the three-dimensional structure of the PeD-J proteins was significantly different compared to other plant lectins, primarily due to the tandem dirigent and jacalin domains. We surveyed the upstream putative promoter regions of the PeD-Js and found that they mainly contained cis-acting elements related to hormone and abiotic stress response. An analysis of the expression patterns of root, leaf, rhizome and panicle revealed that four PeD-J genes were highly expressed in the panicle, indicating that they may be required during the formation and development of several different tissue types in Moso bamboo. Moreover, PeD-J genes were shown to be involved in the rapid growth and development of bamboo shoots. Quantitative Real-time PCR (qRT PCR) assays further verified that D-J family genes were responsive to hormones and stresses. The results of this study will help to elucidate the biological functions of PeD-Js during bamboo growth, development and stress response.
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