Removal of the basement membrane enhances corneal wound healing.
Removal of the basement membrane enhances corneal wound healing.
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DOI：
10.1016/j.exer.2011.10.012
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发表时间：
2011-12
影响因子：
3.4
通讯作者：
Stepp, Mary Ann
中科院分区：
文献类型：
--
作者：
Pal-Ghosh, Sonali;Pajoohesh-Ganji, Ahdeah;Tadvalkar, Gauri;Stepp, Mary Ann
复发的角膜侵蚀是痛苦的，并将患者的视力置于危险之中。治疗通常从清除侵蚀部位周围的区域开始，然后进行更积极的治疗。一种体内的小鼠模型已经被开发出来，该模型可以在使用钝化的刀片创建的一个1.5 mm的角膜清创伤口后的两周内自发地在野生型小鼠中自发地诱导复发的上皮侵蚀。本研究旨在确定1)钝刃伤后愈合过程中抑制基质金属蛋白酶功能是否会影响侵蚀的发展，以及2)旋转毛刺造成的伤口愈合时是否没有侵蚀。钝刃伤后口服或局部抑制MMPs并不能促进愈合。旋转毛刺造成的伤口比钝刃伤口愈合时的侵蚀要少得多。比较两种创伤后基质金属蛋白酶9、β4整合素和基底膜蛋白(LN332和III型胶原)的定位、免疫细胞的流入和角膜神经的再支配。旋转毛刺伤中央去除前基底膜，但不去除创缘附近的外周基底膜，诱导角膜基质细胞更多的凋亡，损伤更多的基质神经纤维。尽管旋转毛刺伤口对角膜的损害更大，但招募的免疫细胞更少，更多的伤口完全愈合。
Recurrent corneal erosions are painful and put patients’ vision at risk. Treatment typically begins with debridement of the area around the erosion site followed by more aggressive treatments. An in vivo mouse model has been developed that reproducibly induces recurrent epithelial erosions in wild-type mice spontaneously within two weeks after a single 1.5 mm corneal debridement wound created using a dulled blade. This study was conducted to determine whether 1) inhibiting MMP function during healing after dulled-blade wounding impacts erosion development and 2) wounds made with a rotating burr heal without erosions. Oral or topical inhibition of MMPs after dulled-blade wounding does not improve healing. Wounds made by rotating burr heal with significantly fewer erosions than dulled-blade wounds. The localization of MMP9, β4 integrin and basement membrane proteins (LN332 and type VII collagen), immune cell influx, and reinnervation of the corneal nerves were compared after both wound types. Rotating-burr wounds remove the anterior basement membrane centrally but not at the periphery near the wound margin, induce more apoptosis of corneal stromal cells, and damage more stromal nerve fibers. Despite the fact that rotating-burr wounds do more damage to the cornea, fewer immune cells are recruited and significantly more wounds resolve completely.
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影响因子：
3.4
作者：
Mohan, Rajiv R.;Stapleton, W. Michael;Wilson, Steven E.
通讯作者：
Wilson, Steven E.
影响因子：
4.4
作者：
Chotikavanich S;de Paiva CS;Li de Q;Chen JJ;Bian F;Farley WJ;Pflugfelder SC
通讯作者：
Pflugfelder SC
影响因子：
2.8
作者：
Aldave, Anthony J.;Kamal, Khairidzan M.;Yu, Fei
通讯作者：
Yu, Fei
影响因子：
4.1
作者：
Mencucci, Rita;Paladini, Iacopo;Romagnoli, Paolo
通讯作者：
Romagnoli, Paolo
影响因子：
4
作者：
Esquenazi, Salomon;He, Jiucheng;Bazan, Haydee E. P.
通讯作者：
Bazan, Haydee E. P.