The Drosophila CD2AP/CIN85 orthologue Cindr regulates junctions and cytoskeleton dynamics during tissue patterning.
The Drosophila CD2AP/CIN85 orthologue Cindr regulates junctions and cytoskeleton dynamics during tissue patterning.
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DOI：
10.1083/jcb.200706108
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发表时间：
2008-03-24
期刊：
影响因子：
--
通讯作者：
Cagan RL
中科院分区：
文献类型：
--
作者：
Johnson RI;Seppa MJ;Cagan RL
发育中的组织需要细胞经历复杂的过程才能转移到合适的生态位。这需要细胞表面的粘附介导事件和细胞骨架重组之间的功能连接，以允许定向运动。少量蛋白质被认为与这些过程有关。在这里，我们确定了一个候选基因Cindr，它是CD2AP/CIN85家族中唯一的黑腹果蝇成员（该家族先前涉及多种过程）。利用黑胃鼠视网膜，我们证明Cindr将细胞表面连接（E-cadherin）和粘附（Roughest）与肌动蛋白细胞骨架的多种成分联系起来。减少煤渣活性导致局部细胞运动缺陷，从而导致组织模式和细胞死亡。Cindr活性对于果蝇E-cadherin和Roughest的正常定位是必需的，并且我们发现了与肌动蛋白细胞骨架的多个组分的额外的物理和功能联系，包括肌动蛋白封盖蛋白和封盖蛋白。总之，这些数据表明Cindr参与新生上皮的动态细胞重排。
Developing tissues require cells to undergo intricate processes to shift into appropriate niches. This requires a functional connection between adhesion-mediating events at the cell surface and a cytoskeletal reorganization to permit directed movement. A small number of proteins are proposed to link these processes. Here, we identify one candidate, Cindr, the sole Drosophila melanogaster member of the CD2AP/CIN85 family (this family has been previously implicated in a variety of processes). Using D. melanogaster retina, we demonstrate that Cindr links cell surface junctions (E-cadherin) and adhesion (Roughest) with multiple components of the actin cytoskeleton. Reducing cindr activity leads to defects in local cell movement and, consequently, tissue patterning and cell death. Cindr activity is required for normal localization of Drosophila E-cadherin and Roughest, and we show additional physical and functional links to multiple components of the actin cytoskeleton, including the actin-capping proteins capping protein alpha and capping protein beta. Together, these data demonstrate that Cindr is involved in dynamic cell rearrangement in an emerging epithelium.
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