Thin slices of teleost retina continue to grow in culture.

Thin slices of teleost retina continue to grow in culture.
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硬骨鱼视网膜薄片在培养物中继续生长。

DOI:
10.1016/0165-0270(91)90045-2
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发表时间:
1991
影响因子:
3
通讯作者:
Fernald,RD
Fernald,RD
中科院分区:
医学4区
文献类型:
--
作者:
Mack,AF;Fernald,RD

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Thin slices of differentiated fish retinas were maintained up to 5 days in culture conditions where they exhibited properties essentially identical to those found in retinas of intact animals. Retinal slices were prepared by embedding eyecups from young fish in agarose and sectioning them on a vibratome. Phenotypic integrity of specific cell types was maintained, as demonstrated by specific antibody staining patterns. Stem cells in the retinal margin and presumptive rod progenitor cells in the outer nuclear layer continued to proliferate in vitro, just as they do in vivo. Some of these cells differentiated in vitro as demonstrated by labelling both cell division and cell phenotype. After several days in culture, some regeneration-like responses were observed, such as growth of neurites and swelling of cell bodies in the ganglion cell layer. This retinal slice preparation appears to offer a unique opportunity for studying the interactions among developing retinal cells.
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