Ebselen Preserves Tissue-Engineered Cell Sheets and their Stem Cells in Hypothermic Conditions.
Ebselen Preserves Tissue-Engineered Cell Sheets and their Stem Cells in Hypothermic Conditions.
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Ebselen在低温条件下保留组织工程的细胞表及其干细胞。
DOI：
10.1038/srep38987
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发表时间：
2016-12-14
影响因子：
4.6
通讯作者：
Nishida K
中科院分区：
文献类型：
--
作者：
Katori R;Hayashi R;Kobayashi Y;Kobayashi E;Nishida K
临床试验已经进行了使用自体组织工程上皮细胞片角膜再生医学。为了改进基于干细胞的治疗以便于临床实践，需要新的技术来保存重建的组织及其干/祖细胞直到它们准备好使用。在本研究中，我们筛选了潜在的防腐剂，并开发了一种新的介质，用于保存细胞片和他们的干/祖细胞的效果进行了评估，与基于酶的活力测定。Nrf 2激活剂，特别是依布硒啉，可以在保存过程中维持高ATP水平。Ebselen还显示出对维持低温下保存的细胞片的活力、形态和干细胞功能的强烈影响，通过保护它们免受活性氧诱导的损伤。此外，依布硒啉大大改善了人类角膜组织及其干细胞的保存性能。因此，依布硒啉显示出良好的潜力，作为一个有用的保存剂，在再生医学以及在角膜移植。
Clinical trials have been performed using autologous tissue-engineered epithelial cell sheets for corneal regenerative medicine. To improve stem cell-based therapy for convenient clinical practice, new techniques are required for preserving reconstructed tissues and their stem/progenitor cells until they are ready for use. In the present study, we screened potential preservative agents and developed a novel medium for preserving the cell sheets and their stem/progenitor cells; the effects were evaluated with a luciferase-based viability assay. Nrf2 activators, specifically ebselen, could maintain high ATP levels during preservation. Ebselen also showed a strong influence on maintenance of the viability, morphology, and stem cell function of the cell sheets preserved under hypothermia by protecting them from reactive oxygen species-induced damage. Furthermore, ebselen drastically improved the preservation performance of human cornea tissues and their stem cells. Therefore, ebselen shows good potential as a useful preservation agent in regenerative medicine as well as in cornea transplantation.
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影响因子：
6.2
作者：
Nishida, K;Yamato, M;Okano, T
通讯作者：
Okano, T
影响因子：
5.8
作者：
BEIL, W;STAAR, U;SEWING, KF
通讯作者：
SEWING, KF
影响因子：
158.5
作者：
Rama, Paolo;Matuska, Stanislav;Pellegrini, Graziella
通讯作者：
Pellegrini, Graziella
影响因子：
--
作者：
MEANS, TL;GEROSKI, DH;EDELHAUSER, HF
通讯作者：
EDELHAUSER, HF
影响因子：
4.4
作者：
Blalock, Timothy D.;Spurr-Michaud, Sandra J.;Gipson, Ilene K.
通讯作者：
Gipson, Ilene K.