Rbf Regulates Drosophila Spermatogenesis via Control of Somatic Stem and Progenitor Cell Fate in the Larval Testis.
Rbf Regulates Drosophila Spermatogenesis via Control of Somatic Stem and Progenitor Cell Fate in the Larval Testis.
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DOI：
10.1016/j.stemcr.2016.11.007
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发表时间：
2016-12-13
影响因子：
5.9
通讯作者：
Hime, Gary R.
中科院分区：
文献类型：
--
作者：
Dominado, Nicole;La Marca, John E.;Siddall, Nicole A.;Heaney, James;Tran, Mai;Cai, Yu;Yu, Fengwei;Wang, Hongyan;Somers, W. Gregory;Quinn, Leonie M.;Hime, Gary R.
果蝇睾丸对于理解干细胞如何与其内源性微环境或生态位相互作用以控制体内器官生长至关重要。在这里，我们报告了两个独立的等位基因的高度保守的肿瘤抑制基因，视网膜母细胞瘤家族蛋白（Rbf），在X染色体三龄致死等位基因的睾丸表型筛选。Rbf突变等位基因表现出精原细胞过度增殖，这是表型模仿的分子特征Rbf11无效等位基因。我们证明，Rbf促进细胞周期退出和睾丸的体细胞和生殖系干细胞的分化。有趣的是，生殖系中特异性的Rbf缺失不会破坏干细胞分化，相反，体细胞谱系中Rbf功能的丧失会驱动两种谱系中的过度增殖和分化缺陷。总之，我们的观察表明，Rbf在体细胞谱系控制生殖系干细胞的更新和分化非自主通过在生殖系谱系的微环境中的重要作用。Rbf缺失睾丸表现出生殖系干细胞分化失败，L3睾丸体细胞中的Rbf表达挽救了GSC分化缺陷体细胞Rbf RNAi破坏了包囊干细胞和生殖系干细胞分化体细胞E2f1的耗竭挽救了Rbf生殖系增殖和分化果蝇睾丸包含生殖系和体细胞包囊干细胞群体。来自这些种群的后代交叉调节每个谱系的分化。在这篇文章中，Hime、Quinn及其同事表明，果蝇视网膜母细胞瘤直系同源物Rbf是睾丸体细胞自主需要的细胞，以促进囊肿干细胞分化和随后的种系干细胞分化。
The Drosophila testis has been fundamental to understanding how stem cells interact with their endogenous microenvironment, or niche, to control organ growth in vivo. Here, we report the identification of two independent alleles for the highly conserved tumor suppressor gene, Retinoblastoma-family protein (Rbf), in a screen for testis phenotypes in X chromosome third-instar lethal alleles. Rbf mutant alleles exhibit overproliferation of spermatogonial cells, which is phenocopied by the molecularly characterized Rbf11 null allele. We demonstrate that Rbf promotes cell-cycle exit and differentiation of the somatic and germline stem cells of the testes. Intriguingly, depletion of Rbf specifically in the germline does not disrupt stem cell differentiation, rather Rbf loss of function in the somatic lineage drives overproliferation and differentiation defects in both lineages. Together our observations suggest that Rbf in the somatic lineage controls germline stem cell renewal and differentiation non-autonomously via essential roles in the microenvironment of the germline lineage. Rbf null testes exhibit failure of germline stem cells to differentiate Rbf expression in somatic cells of L3 testes rescues the GSC differentiation defect Somatic Rbf RNAi disrupts cyst stem cell and germline stem cell differentiation Somatic depletion of E2f1 rescues Rbf germline proliferation and differentiation The Drosophila testis contains germline and somatic cyst stem cell populations. Progeny from these populations cross regulate the differentiation of each lineage. In this article, Hime, Quinn, and colleagues show that the Drosophila Retinoblastoma ortholog, Rbf, is cell autonomously required by somatic cells of the testis to facilitate cyst stem cell differentiation and subsequent germline stem cell differentiation.
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DOI：
10.1073/pnas.1311548110
发表时间：
2013-07-30
影响因子：
11.1
作者：
Hu, Yueh-Chiang;de Rooij, Dirk G.;Page, David C.
通讯作者：
Page, David C.
影响因子：
64.5
作者：
deNooij, JC;Letendre, MA;Hariharan, IK
通讯作者：
Hariharan, IK
影响因子：
64.8
作者：
Kiger, AA;White-Cooper, H;Fuller, MT
通讯作者：
Fuller, MT
影响因子：
8.8
作者：
Gonzalez J;Qi H;Liu N;Lin H
通讯作者：
Lin H
影响因子：
10.5
作者：
JOHNSON, DG;OHTANI, K;NEVINS, JR
通讯作者：
NEVINS, JR