Tomographic phase microscopy: principles and applications in bioimaging [Invited].
Tomographic phase microscopy: principles and applications in bioimaging [Invited].
复制标题
层析成像阶段显微镜:生物成像中的原理和应用[邀请]。
DOI:
10.1364/josab.34.000b64
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发表时间:
2017
期刊:
影响因子:
--
通讯作者:
So PTC
中科院分区:
文献类型:
--
作者:
Jin D;Zhou R;Yaqoob Z;So PTC
Tomographic phase microscopy (TPM) is an emerging optical microscopic technique for bioimaging. TPM uses digital holographic measurements of complex scattered fields to reconstruct three-dimensional refractive index (RI) maps of cells with diffraction-limited resolution by solving inverse scattering problems. In this paper, we review the developments of TPM from the fundamental physics to its applications in bioimaging. We first provide a comprehensive description of the tomographic reconstruction physical models used in TPM. The RI map reconstruction algorithms and various regularization methods are discussed. Selected TPM applications for cellular imaging, particularly in hematology, are reviewed. Finally, we examine the limitations of current TPM systems, propose future solutions, and envision promising directions in biomedical research.
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