Relative biological effects of neutron mixed-beam irradiation for boron neutron capture therapy on cell survival and DNA double-strand breaks in cultured mammalian cells.
Relative biological effects of neutron mixed-beam irradiation for boron neutron capture therapy on cell survival and DNA double-strand breaks in cultured mammalian cells.
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DOI：
10.1093/jrr/rrs079
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发表时间：
2013-01
影响因子：
2
通讯作者：
Takahashi S
中科院分区：
文献类型：
--
作者：
Okumura K;Kinashi Y;Kubota Y;Kitajima E;Okayasu R;Ono K;Takahashi S
了解用于硼中子俘获疗法 (BNCT) 的中子混合束辐照的生物学效应对于提高治疗效果并减少副作用非常重要。在本研究中，在中子混合束照射 BNCT 后，检查了中国仓鼠卵巢细胞 (CHO-K1) 及其放射敏感性突变细胞（xrs5、Ku80 缺陷）的细胞活力和 DNA 双链断裂 (DNA-DSB)。与参考伽马射线照射组相比，中子照射组的细胞活力显着受损。 CHO-K1 和 xrs5 细胞的 10% 细胞存活率的相对生物有效性分别为 3.3 和 1.2。在中子混合束和伽马射线组中，有相似数量的 53BP1 焦点（DNA-DSB 的指标）。此外，各组之间焦点的大小没有差异。然而，中子混合束辐照导致焦点具有不同的空间分布。中子混合束组中的焦点比伽马射线照射组中的焦点彼此更接近。这些发现表明，中子束可能会诱发另一种类型的 DNA 损伤，例如簇状 DNA-DSB，正如其他高 LET 辐射所表明的那样。
Understanding the biological effects of neutron mixed-beam irradiation used for boron neutron capture therapy (BNCT) is important in order to improve the efficacy of the therapy and to reduce side effects. In the present study, cell viability and DNA double-strand breaks (DNA-DSBs) were examined in Chinese hamster ovary cells (CHO-K1) and their radiosensitive mutant cells (xrs5, Ku80-deficient), following neutron mixed-beam irradiation for BNCT. Cell viability was significantly impaired in the neutron irradiation groups compared to the reference gamma-ray irradiation group. The relative biological effectiveness for 10% cell survival was 3.3 and 1.2 for CHO-K1 and xrs5 cells, respectively. There were a similar number of 53BP1 foci, indicators of DNA-DSBs, in the neutron mixed-beam and the gamma-ray groups. In addition, the size of the foci did not differ between groups. However, neutron mixed-beam irradiation resulted in foci with different spatial distributions. The foci were more proximal to each other in the neutron mixed-beam groups than the gamma-ray irradiation groups. These findings suggest that neutron beams may induce another type of DNA damage, such as clustered DNA-DSBs, as has been indicated for other high-LET irradiation.
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影响因子：
2.6
作者：
GOODHEAD, DT
通讯作者：
GOODHEAD, DT
影响因子：
3.4
作者：
Johnston, CJ;Williams, JP;Finkelstein, JN
通讯作者：
Finkelstein, JN
影响因子：
5.3
作者：
Singleton, BK;Priestley, A;Jeggo, PA
通讯作者：
Jeggo, PA
影响因子：
3.4
作者：
Cromheecke, M;Piers, BA;Hoekstra, HJ
通讯作者：
Hoekstra, HJ
影响因子：
2
作者：
Antoccia, Antonio;Sgura, Antonella;Tanzarella, Caterina
通讯作者：
Tanzarella, Caterina