Single-nucleus transcriptomes reveal evolutionary and functional properties of cell types in the Drosophila accessory gland.
Single-nucleus transcriptomes reveal evolutionary and functional properties of cell types in the Drosophila accessory gland.
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单核转录组揭示了果蝇副腺细胞类型的进化和功能特性。
DOI：
10.1093/genetics/iyab213
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发表时间：
2022
期刊：
影响因子：
3.3
通讯作者：
Begun,DavidJ
中科院分区：
文献类型：
--
作者：
Majane,AlexC;Cridland,JulieM;Begun,DavidJ
许多负责雄性生殖的性状进化很快，包括生殖系和体细胞雄性生殖组织中的基因表达表型。一夫多妻制物种中的快速雄性进化被认为是由雄性之间对受精的竞争以及雄性和雌性适合性利益之间的冲突所推动的，这些冲突表现在交配后的表型上。在果蝇中，雄性附腺和射精管三种主要细胞分泌的精液蛋白是雌性精子储存和使用所必需的，并影响雌性交配后的特征。最近的研究表明，这些细胞类型对雌性交配后的生物学有重叠但不同的影响，但人们对它们的进化特性知之甚少。在这里，我们首次利用果蝇和两个近缘物种的副腺和射精管的单核RNA-Seq来全面描述这些组织的细胞多样性及其转录组进化。我们发现，精液转录本在主要细胞类型中被强烈分割，许多其他基因的表达额外定义了每种细胞类型。我们还报告了以前未被证实的主细胞的多样性。转录组的差异被发现在细胞类型和谱系之间是不同的，揭示了一个复杂的进化过程。此外，不同细胞类型的蛋白质适应性不同，这可能会影响我们对雄性交配后性状选择的理解。
Many traits responsible for male reproduction evolve quickly, including gene expression phenotypes in germline and somatic male reproductive tissues. Rapid male evolution in polyandrous species is thought to be driven by competition among males for fertilizations and conflicts between male and female fitness interests that manifest in postcopulatory phenotypes. InDrosophila, seminal fluid proteins secreted by three major cell types of the male accessory gland and ejaculatory duct are required for female sperm storage and use, and influence female postcopulatory traits. Recent work has shown that these cell types have overlapping but distinct effects on female postcopulatory biology, yet relatively little is known about their evolutionary properties. Here, we use single-nucleus RNA-Seq of the accessory gland and ejaculatory duct fromDrosophila melanogasterand two closely related species to comprehensively describe the cell diversity of these tissues and their transcriptome evolution for the first time. We find that seminal fluid transcripts are strongly partitioned across the major cell types, and expression of many other genes additionally defines each cell type. We also report previously undocumented diversity in main cells. Transcriptome divergence was found to be heterogeneous across cell types and lineages, revealing a complex evolutionary process. Furthermore, protein adaptation varied across cell types, with potential consequences for our understanding of selection on male postcopulatory traits.
影响因子：
--
作者：
M. Campanacci;M. Laus
通讯作者：
M. Laus
通过球心全膝关节置换术和自体高压灭菌骨移植对膝关节肿瘤进行保肢手术。
影响因子：
4.2
作者：
J. Johnston;T. Harries;C. E. Alexander;A. Alexander
通讯作者：
A. Alexander