Flow cytometric estimation of transmembrane potential of macrophages--a comparison with microelectrode measurements.
Flow cytometric estimation of transmembrane potential of macrophages--a comparison with microelectrode measurements.
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巨噬细胞跨膜电位的流式细胞术估计——与微电极测量的比较。
DOI：
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
E. Mix
中科院分区：
文献类型：
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作者：
H. Jenssen;K. Redmann;E. Mix
研究了亲脂性阳离子染料3，3 '二己基氧碳菁（DiOC 6（3））在巨噬细胞中的电位依赖性蓄积。通过流式细胞术测量细胞的所得荧光。离子载体处理（缬氨霉素和短杆菌肽）以剂量依赖性（10（-5）M-10（-7）M）和时间依赖性（0 min-45 min）方式诱导巨噬细胞膜电位的改变。将相对荧光强度的变化与通过应用玻璃微电极获得的细胞内记录测量的跨膜电位的变化进行比较。比较研究提供了校准悬浮细胞膜电位的流式细胞仪估计的可能性。在5 × 10（-8）M和10（-7）M之间的染料浓度和添加染料后10 min至30 min的孵育间隔内，细胞和周围培养基之间染料分配的平衡严格依赖于电位。得出结论的光学方法的应用领域。从改变离子传输的分子机制的角度讨论了离子载体处理后电过程的动力学。
Potential-dependent accumulation of the lipophilic cationic dye 3,3' dihexyloxacarbocyanine (DiOC6(3)) in macrophages has been investigated. Resulting fluorescence of cells was measured by flow cytometry. Alterations of membrane potential of macrophages were induced by ionophore treatment (valinomycin and gramicidin) in a dose-dependent (10(-5) M-10(-7) M) and time-dependent (0 min-45 min) manner. Resulting changes in relative fluorescence intensity were compared with changes of transmembrane potential measured by intracellular recordings obtained by applying glass microelectrodes. The comparative studies offer the possibility to calibrate the flow cytometric estimate of membrane potential of suspended cells. Equilibration of dye partition between cells and surrounding medium is strictly potential-dependent at dye concentrations between 5 X 10(-8) M and 10(-7) M and within an incubation interval from 10 min up to 30 min after addition of dye. Conclusions are drawn concerning the field of application of the optical method. Dynamics of electrical processes following ionophore treatment are discussed in terms of molecular mechanisms of altered ionic transport.
DOI：
10.1002/cyto.990030402
发表时间：
1983-01-01
期刊：
CYTOMETRY
影响因子：
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作者：
SHAPIRO, HM
通讯作者：
SHAPIRO, HM
使用离子探针表征 II 型肺泡细胞的血浆和线粒体膜电位。
DOI：
10.1016/0005-2736(84)90536-4
发表时间：
1984
期刊：
Biochimica et biophysica acta
影响因子：
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作者：
Gallo,RL;Finkelstein,JN;Notter,RH
通讯作者：
Notter,RH