Identification of spinach SIAMESE and analysis of its function in plant immunity
Identification of spinach SIAMESE and analysis of its function in plant immunity
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菠菜暹罗的鉴定及其植物免疫功能分析
DOI：
10.1080/07060661.2017.1347202
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发表时间：
2017-04
影响因子：
2
通讯作者：
Wang Shui
中科院分区：
文献类型：
--
作者：
Wang Tianhua;Song Teng;Xu Chenxi;Cai Xiaofeng;Wang Xiaoli;Ma Huimin;Zhou Jianjian;Ge Chenhui;Wang Quanhua;Wang Shui
摘要病害对菠菜的产量和品质都有很大的影响。细胞周期信号通路在平衡植物的发育和免疫中起着核心作用。细胞周期蛋白依赖性激酶抑制剂（CKI）是一种核心细胞周期调节剂。已经发现两种拟南芥CKIs，SIAMESE（SIM）和SMR 1（SIAMESE-Related 1），冗余地不仅作为细胞增殖的负调节剂，而且作为植物免疫的正调节剂。在菠菜基因组中，我们鉴定了拟南芥SIM的同源物，称为菠菜SIAMESE（SoSIM）。为了研究SoSIM的功能，我们将35 S启动子驱动的SoSIM（35 S：SoSIM）导入拟南芥sim smr 1双突变体中。SoSIM的过表达表型干扰了细胞周期蛋白依赖性激酶A1（CDKA 1）的RNA干扰，表现出矮小和锯齿状的叶片，证实了SIM是CDK抑制剂。拟南芥野生型的毛状体是单细胞的，而sim smr 1突变体的毛状体是多细胞的。SoSIM恢复了sim smr 1突变体的野生型毛状体表型。sim smr 1突变体对无毒病原菌假单胞菌pv. maculicola与野生型植物相比。我们的数据表明，转35 S：SoSIM基因的sim smr 1株系对病原菌的抗性完全恢复，表明SoSIM激活了植物免疫。这些数据验证了SoSIM在抑制植物发育和激活植物免疫方面与拟南芥对应物一样起作用。因此，可探索SoSIM调控菠菜生长发育与免疫平衡的途径。
Abstract Diseases cause a significant loss in both yield and quality of spinach. The cell cycle signalling pathway plays a central role in balancing development and immunity of plants. Cyclin-Dependent Kinase Inhibitor (CKI) is a core cell cycle regulator. It has been found that two Arabidopsis CKIs, SIAMESE (SIM) and SMR1 (SIAMESE-Related 1), function redundantly not only as negative regulators of cell proliferation but also as positive regulators of plant immunity. In the spinach genome, we identified a homologue of Arabidopsis SIM, referred to as Spinacia oleracea SIAMESE (SoSIM). To investigate the function of SoSIM, we introduced the 35S promoter-driven SoSIM (35S:SoSIM) into Arabidopsis sim smr1 double mutants. Over-expression of SoSIM phenocopied RNA interference of Cyclin-Dependent Kinase A1 (CDKA1) which exhibited dwarf and serrated leaves, confirming that SIM is a CDK inhibitor. Arabidopsis wild-type trichomes are single and unicellular, whereas sim smr1 mutant trichomes are clustered and multicellular. SoSIM restored wild-type trichome phenotype of sim smr1 mutant. The sim smr1 mutants were susceptible to the avirulent pathogen Pseudomonas syringae pv. maculicola as compared with wild-type plants. Our data showed that the resistance of 35S:SoSIM-transgenic sim smr1 lines to the pathogen was fully restored, indicating that SoSIM activates plant immunity. These data verify that SoSIM functions as its Arabidopsis counterpart in inhibition of plant development and activation of plant immunity. Therefore, SoSIM can be explored to control the balance between development and immunity in spinach.
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人类 p53 对植物发育的影响
影响因子：
3.7
作者：
Ma Huimin;Song Teng;Wang Tianhua;Wang Shui
通讯作者：
Wang Shui
影响因子：
7.4
作者：
Aurine Verkest;C. Weinl;D. Inzé;L. De Veylder;A. Schnittger
通讯作者：
Aurine Verkest;C. Weinl;D. Inzé;L. De Veylder;A. Schnittger
影响因子：
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作者：
J. E. Bamji;N. Bamji
通讯作者：
J. E. Bamji;N. Bamji
影响因子：
30.3
作者：
Wang S;Gu Y;Zebell SG;Anderson LK;Wang W;Mohan R;Dong X
通讯作者：
Dong X
DOI：
10.1046/j.1365-313x.2000.00688.x
发表时间：
2000-02
期刊：
The Plant journal : for cell and molecular biology
影响因子：
--
作者：
Helen Lui;Hong Wang;Catherine DeLong;L. Fowke;William L. Crosby;Pierre R. Fobert
通讯作者：
Helen Lui;Hong Wang;Catherine DeLong;L. Fowke;William L. Crosby;Pierre R. Fobert