Large-scale investigation of the olfactory receptor space using a microfluidic microwell array.
Large-scale investigation of the olfactory receptor space using a microfluidic microwell array.
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DOI：
10.1039/b920585c
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发表时间：
2010-05-07
期刊：
影响因子：
6.1
通讯作者：
Folch A
中科院分区：
文献类型：
--
作者：
Figueroa XA;Cooksey GA;Votaw SV;Horowitz LF;Folch A
哺乳动物的嗅觉系统能够区分成千上万的气味分子。在小鼠中，每一种气味都是由大约1,000种气味受体（OR）类型中的一小部分来感知的，每个嗅觉感觉神经元（OSN）都表达一个OR基因。然而，OR/OSN类型的大量库的总和和异源表达的困难使得几乎不可能分析所有OR/OSN类型的气味响应性。我们开发了一种微流体方法，使我们能够在微孔中一次筛选超过20，000个单细胞。通过使用钙成像，我们能够同时检测和分析约2，900个OSN的气味反应。重要的是，该技术允许检测罕见的响应OSN以及鉴定对不相关结构的气味剂广泛响应的OSN群体。这项技术通常适用于筛选大量的单细胞，并应有助于在毒理学，药理学和癌症研究等领域表征罕见的细胞行为。
The mammalian olfactory system is able to discriminate among tens of thousands of odorant molecules. In mice, each odorant is sensed by a small subset of the approximately 1,000 odorant receptor (OR) types, with one OR gene expressed by each olfactory sensory neuron (OSN). However, the sum of the large repertoire of OR/OSN types and difficulties with heterologous expression have made it almost impossible to analyze odorant responsiveness across all OR/OSN types. We have developed a microfluidic approach that allowed us to screen over 20,000 single cells at once in microwells. By using calcium imaging, we were able to detect and analyze odorant responses of about 2,900 OSNs simultaneously. Importantly, this technique allows for both the detection of rare responding OSNs as well as the identification of OSN populations broadly responsive to odorants of unrelated structures. This technique is generally applicable for screening large numbers of single cells and should help to characterize rare cell behaviors in fields such as toxicology, pharmacology, and cancer research.
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小鼠嗅觉神经元的快速适应不需要磷酸二酯酶的活性。
影响因子：
3.8
作者：
Boccaccio, Anna;Lagostena, Laura;Hagen, Volker;Menini, Anna
通讯作者：
Menini, Anna
影响因子：
16.2
作者：
Friedrich, RW;Korsching, SI
通讯作者：
Korsching, SI
影响因子：
1.7
作者：
Dorries, KM;AdkinsRegan, E;Halpern, BP
通讯作者：
Halpern, BP
DOI：
10.1101/sqb.1992.057.01.056
发表时间：
1992-01-01
期刊：
COLD SPRING HARBOR SYMPOSIA ON QUANTITATIVE BIOLOGY
影响因子：
--
作者：
CHESS, A;BUCK, L;NGAI, J
通讯作者：
NGAI, J
影响因子：
5.5
作者：
Araneda, RC;Peterlin, Z;Firestein, S
通讯作者：
Firestein, S