Sense and anti-sense transcription in the murine c-myb attenuator region.
Sense and anti-sense transcription in the murine c-myb attenuator region.
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小鼠 c-myb 衰减区中的有义和反义转录。
DOI：
10.1007/978-3-642-74006-0_43
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发表时间：
1988
影响因子：
--
通讯作者：
C. Thompson
中科院分区：
文献类型：
--
作者：
T. Bender;K. Catron;W. Kuehl;C. Thompson
已发现c-myb原癌基因主要在正常组织和未成熟造血来源的肿瘤细胞系中高水平表达（Westin 1982; Gonda 1982; Duprey 1985; Bender 1987a）。在每个谱系中，c-myb mRNA的表达在成熟过程中都有所下降。最近的证据表明，至少在T细胞谱系中，c-myb mRNA的表达可能从未成熟T细胞中的高组成表达转变为更成熟发育阶段的细胞周期相关表达模式（Thompson 1986; Reed 1986）。这些发现表明，c-myb基因产物可能在造血成熟中发挥作用，并且c-myb表达的下调是正常发育进行所必需的。事实上，转染实验已经证明myb的组成表达可能会干扰tsE26转化的单核细胞的分化和小鼠红细胞白血病细胞的诱导分化（Beug 1987; Clarke 1988）。我们已经开始研究如何在一系列小鼠B淋巴样肿瘤中确定c-myb稳态mRNA水平，这些肿瘤显示c-myb mRNA水平的发育调节表达。在这些肿瘤细胞系中，前B细胞淋巴瘤的c-myb mRNA含量是B细胞淋巴瘤或浆细胞瘤的10至100倍以上（Bender 1987a）。我们发现这些稳定状态c-myb mRNA水平的差异主要是由c-myb位点第一个内含子的转录延伸阻滞调节的（Bender 1987b）。
The c-myb proto-oncogene has been found to be expressed at high levels predominantly in normal tissues and tumor cell lines of immature hematopoietic origin (Westin 1982; Gonda 1982; Duprey 1985; Bender 1987a). In each lineage which has been examined expression of c-myb mRNA has been found to decrease during maturation. Recent evidence suggests, at least in the T cell lineage, that expression of c-myb mRNA may switch from high constitutive expression in immature T cells to a cell cycle related mode of expression at more mature stages of development (Thompson 1986; Reed 1986). These findings have suggested that the c-myb gene product may play a role in hematopoietic maturation and that down-regulation of c-myb expression is required for normal development to proceed. Indeed, transfection experiments have demonstrated that constitutive expression of myb may interfere with differentiation in tsE26 transformed monocytic cells and the inducible differentiation of murine erythroleukemia cells (Beug 1987; Clarke 1988). We have begun to examine how c-myb steady state mRNA levels are determined in a series of murine B lymphoid tumors which demonstrate developmentally regulated expression of c-myb mRNA levels. In these tumor cell lines, pre-B cell lymphomas contain 10 to greater than 100 times as much c-myb mRNA than B cell lymphomas or plasmacytomas (Bender 1987a). We find that these differences in steady state c-myb mRNA levels are regulated primarily by a block to transcription elongation which occurs in the first intron of the c-myb locus (Bender 1987b).
体内和体外腺病毒转录过程中人 RNA 聚合酶 II 的暂停和提前终止。
DOI：
10.1073/pnas.81.19.5931
发表时间：
1984
影响因子：
11.1
作者：
Maderious,A;Chen-Kiang,S
通讯作者：
Chen-Kiang,S
影响因子：
56.9
作者：
STERN, JB;SMITH, KA
通讯作者：
SMITH, KA