Comprehensive Analysis of hsa-miR-654-5p’s Tumor-Suppressing Functions
Comprehensive Analysis of hsa-miR-654-5p’s Tumor-Suppressing Functions
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hsa-miR-654-5p的抑癌功能综合分析
DOI：
10.3390/ijms23126411
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发表时间：
2022-06-08
影响因子：
5.6
通讯作者：
Zhu, Lingyun
中科院分区：
文献类型：
--
作者：
Liu, Chuanyang;Min, Lu;Kuang, Jingyu;Zhu, Chushu;Qiu, Xinyuan;Wu, Xiaomin;Zhang, Tianyi;Xie, Sisi;Zhu, Lingyun
近年来，miRNAs在多种癌症的发生、转移和预后中起着关键作用。本研究旨在探讨hsa-miR-654-5p在肺癌中的具体作用。将一系列闭环生物信息学功能分析与体外实验验证相结合，探索miR-654-5P的整体生物学功能和泛癌调控模式。我们发现，在LUAD组织中miR-654-5p的丰度显著升高，并与患者的生存相关。然后确定了miR-654-5P的275个潜在靶点，并在体外验证了miR-654-5P-RNF8调节轴的概念。此外，我们揭示了miR-654-5p的肿瘤抑制作用，并通过基于靶点、基于丰度和基于ssGSEA的生物信息学方法和体外验证证明了miR-654-5p抑制肺癌细胞上皮-间充质转化(EMT)过程、细胞增殖和迁移。在构建蛋白质-蛋白质相互作用网络后，确定了11个高度互联的HUB基因，并建立了5个基因的风险评分模型来评估它们的潜在预后能力。我们的研究不仅为了解miR-654-5p在不同肿瘤中的功能提供了一个基本的miRNA-mRNA表型参考图，而且揭示了miR-654-5p的抑瘤作用和预后价值。
The pivotal roles of miRNAs in carcinogenesis, metastasis, and prognosis have been demonstrated recently in various cancers. This study intended to investigate the specific roles of hsa-miR-654-5p in lung cancer, which is, in general, rarely discussed. A series of closed-loop bioinformatic functional analyses were integrated with in vitro experimental validation to explore the overall biological functions and pan-cancer regulation pattern of miR-654-5p. We found that miR-654-5p abundance was significantly elevated in LUAD tissues and correlated with patients’ survival. A total of 275 potential targets of miR-654-5p were then identified and the miR-654-5p-RNF8 regulation axis was validated in vitro as a proof of concept. Furthermore, we revealed the tumor-suppressing roles of miR-654-5p and demonstrated that miR-654-5p inhibited the lung cancer cell epithelial-mesenchymal transition (EMT) process, cell proliferation, and migration using target-based, abundance-based, and ssGSEA-based bioinformatic methods and in vitro validation. Following the construction of a protein–protein interaction network, 11 highly interconnected hub genes were identified and a five-genes risk scoring model was developed to assess their potential prognostic ability. Our study does not only provide a basic miRNA-mRNA-phenotypes reference map for understanding the function of miR-654-5p in different cancers but also reveals the tumor-suppressing roles and prognostic values of miR-654-5p.
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影响因子：
3.9
作者：
Kong, Rui
通讯作者：
Kong, Rui
RNF8促进乳腺癌细胞上皮间质转化
DOI：
10.1186/s13046-016-0363-6
发表时间：
2016-06-04
期刊：
Journal of experimental & clinical cancer research : CR
影响因子：
--
作者：
Kuang J;Li L;Guo L;Su Y;Wang Y;Xu Y;Wang X;Meng S;Lei L;Xu L;Shao G
通讯作者：
Shao G
影响因子：
4.4
作者：
Koch A;De Meyer T;Jeschke J;Van Criekinge W
通讯作者：
Van Criekinge W
乳腺癌中 miR-622 关键靶基因的生物信息学鉴定和 miR-622-RNF8 轴的实验验证
影响因子：
4.7
作者：
Liu, Chuanyang;Min, Lu;Zhu, Lingyun
通讯作者：
Zhu, Lingyun
影响因子：
8
作者：
Majem, Blanca;Parrilla, Alfonso;Santamaria, Anna
通讯作者：
Santamaria, Anna