Efficacy of antibody-based therapies against Middle East respiratory syndrome coronavirus (MERS-CoV) in common marmosets.
Efficacy of antibody-based therapies against Middle East respiratory syndrome coronavirus (MERS-CoV) in common marmosets.
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DOI：
10.1016/j.antiviral.2017.03.025
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发表时间：
2017-07
影响因子：
7.6
通讯作者：
Munster VJ
中科院分区：
文献类型：
--
作者：
van Doremalen N;Falzarano D;Ying T;de Wit E;Bushmaker T;Feldmann F;Okumura A;Wang Y;Scott DP;Hanley PW;Feldmann H;Dimitrov DS;Munster VJ
中东呼吸综合征冠状病毒 (MERS-CoV) 病例不断被发现，由于缺乏有效的临床治疗和预防策略，使用恢复期血浆或单克隆抗体 (mAb) 进行治疗是一种潜在的快速干预途径。通过恢复期血浆或单克隆抗体进行的被动免疫疗法已被证明对其他传染源有效。感染 MERS-CoV 后，在接种后 6 小时和 48 小时用高效价超免疫血浆或 mAb m336 处理普通狨猴。两种治疗均减少了临床疾病的体征，但仅在超免疫血浆组中发现呼吸道病毒载量减少。仅在单克隆抗体治疗组中发现了总体病理学的减少，但在治疗组和对照动物之间没有观察到组织学差异。虽然超免疫血浆和 m336 治疗都降低了普通狨猴疾病的严重程度，但这两种治疗都无法完全预防疾病。用 m336 或超免疫血浆治疗感染 MERS-CoV 的普通狨猴可减少临床疾病的迹象。只有用超免疫血浆治疗才能减少病毒载量。仅用单克隆抗体 m336 治疗可导致总体病理学减少。未处理和处理的普通狨猴之间没有观察到组织学差异。
Cases of Middle East respiratory syndrome coronavirus (MERS-CoV) continue to be identified and with a lack of effective clinical treatment and no preventative strategies, treatment using convalescent plasma or monoclonal antibodies (mAbs) is a potential quick route to an intervention. Passive immunotherapy via either convalescent plasma or mAbs has proven to be effective for other infectious agents. Following infection with MERS-CoV, common marmosets were treated with high titer hyperimmune plasma or the mAb m336, at 6 and 48 h post inoculation. Both treatments reduced signs of clinical disease, but reduction in viral loads in the respiratory tract were only found in the hyperimmune plasma group. A decrease in gross pathology was found only in the mAb-treated group, but no histological differences were observed between treated and control animals. While both hyperimmune plasma and the m336 treatments reduced the severity of disease in the common marmoset, neither treatment resulted in full protection against disease. Treatment of MERS-CoV-infected common marmosets with either m336 or hyperimmune plasma reduced signs of clinical disease. Only treatment with hyperimmune plasma resulted in reduced viral load. Only treatment with monoclonal antibody m336 resulted in reduced gross pathology. No histological differences were observed between untreated and treated common marmosets.
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影响因子：
6.7
作者：
Falzarano D;de Wit E;Feldmann F;Rasmussen AL;Okumura A;Peng X;Thomas MJ;van Doremalen N;Haddock E;Nagy L;LaCasse R;Liu T;Zhu J;McLellan JS;Scott DP;Katze MG;Feldmann H;Munster VJ
通讯作者：
Munster VJ
DOI：
10.1007/s10096-004-1271-9
发表时间：
2005-01
期刊：
European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology
影响因子：
--
作者：
Cheng Y;Wong R;Soo YO;Wong WS;Lee CK;Ng MH;Chan P;Wong KC;Leung CB;Cheng G
通讯作者：
Cheng G
DOI：
10.1073/pnas.1222878110
发表时间：
2013-02-26
影响因子：
11.1
作者：
Seok, Junhee;Warren, H. Shaw;Tompkins, Ronald G.
通讯作者：
Tompkins, Ronald G.
DOI：
10.1073/pnas.1510199112
发表时间：
2015-08-18
影响因子：
11.1
作者：
Corti, Davide;Zhao, Jincun;Lanzavecchia, Antonio
通讯作者：
Lanzavecchia, Antonio
影响因子：
17.1
作者：
Luke, Thomas;Wu, Hua;Frieman, Matthew B.
通讯作者：
Frieman, Matthew B.